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Ability of Oral Bacteria to Induce Tissue-destructive Molecules from Human Neutrophils

Cited 24 time in Web of Science Cited 25 time in Scopus
Authors
Shin, J; Ji, S; Choi, Y
Issue Date
2008
Publisher
John Wiley & Sons
Citation
Oral Diseases (2008) 14:327–334
Keywords
neutrophilsoral bacteriaphagocytosistissue-destructive molecules
Abstract
Aim: The induction of tissue-destructive molecules from neutrophils by periodontopathic bacteria has been suggested as one of the mechanisms of periodontal destruction. The aim of this study was to determine whether the ability to stimulate neutrophils is an authentic characteristic of periodontopathic bacteria.

Methods: We evaluated, along with phagocytosis, the production of reactive oxygen species (ROS), matrix metalloproteinase-8 (MMP-8), and interleukin-1β by neutrophils in response to non-periodontopathic Streptococcus sanguinis and periodontopathic bacteria Fusobacterium nucleatum and Treponema denticola, in the absence or presence of antibodies. Phagocytosis, the death of neutrophils, and intracellular ROS production were measured by flow cytometry and the concentrations of MMP-8 and interleukin-1β secreted into medium were determined by enzyme-linked immunosorbent assay.

Results: S. sanguinis and F. nucleatum induced greater production of ROS, MMP-8, and interleukin-1β than did T. denticola. The levels of tissue-destructive molecules produced by neutrophils had a positive correlation with phagocytosis. Opsonization of bacteria with antibodies significantly increased phagocytosis and ROS production and release, thus increasing both bacterial clearance and potential tissue damage.

Conclusion: The ability of oral bacteria to induce tissue-destructive molecules from neutrophils is not an inherent characteristic of periodontopathic bacteria, which would provide a new insight into the role of neutrophils in periodontal destruction.
ISSN
1354-523X
Language
English
URI
https://hdl.handle.net/10371/69776
DOI
https://doi.org/10.1111/j.1601-0825.2007.01382.x
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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