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Human Hertwigs epithelial root sheath cells play crucial roles in cementum formation

Cited 98 time in Web of Science Cited 101 time in Scopus
Authors
Seo, B-M; Sonoyama, W; Yamaza, T; Shi, S
Issue Date
2007-07
Publisher
American Association for Dental Research/American Academy of Implant Dentistry
Citation
Journal of Dental Research 86:594-599
Keywords
Hertwig`s epithelial root sheath cellperiodontal ligament stem cellepithelial mesenchymal transitioncementum formation
Abstract
Hertwig's epithelial root sheath (HERS) cells are a unique population of epithelial cells in the periodontal ligament compartment. To date, their functional role has not been fully elucidated. Our hypothesis was that HERS cells may be involved in regulating differentiation of periodontal ligament stem cells (PDLSCs) and forming cementum in vivo. In this study, we found that HERS cells may be capable of promoting PDLSC differentiation and undergoing epithelial-mesenchymal transition in vitro. Immunohistochemical staining, Western blot analysis, a transwell co-culture system, and in vivo transplantation were used to characterize the interplay between HERS cells and PDLSCs, as well as the epithelial-mesenchymal transition (EMT) of HERS cells. TGFβ1 was capable of inducing the epithelial-mesenchymal transition of HERS cells through activating the PI3K/AKT pathway. Furthermore, HERS cells were able to form cementum-like tissue when transplanted into immunocompromised mice. Abbreviations: bone marrow mesenchymal stem cell, BMMSC; bone sialoprotein, BSP; hydroxyapatite/tricalcium phosphate, HA/TCP; Hertwig's epithelial root sheath, HERS; osteocalcin, OCN; periodontal ligament, PDL; periodontal ligament stem cell, PDLSC; phosphatidylinositol 3-kinase, PI3K.
KEY WORDS: Hertwig's epithelial root sheath cell, periodontal ligament stem cell; epithelial-mesenchymal transition, cementum formation.
ISSN
0022-0345
Language
English
URI
https://hdl.handle.net/10371/69783
DOI
https://doi.org/10.1177/154405910708600703
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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