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Innate immune responses of gingival epithelial cells to nonperiodontopathic and periodontopathic bacteria

Cited 79 time in Web of Science Cited 81 time in Scopus
Authors
Ji, S; Kim, Y; Min, B.-M; Han, S. H; Choi, Y
Issue Date
2007-04
Publisher
Wiley-Blackwell
Citation
JOURNAL OF PERIODONTAL RESEARCH 2007;42:503-510
Keywords
epitheliuminnate immunitynonperiodontopathic bacteriaperiodontal pathogens
Abstract
Background and Objective: We have previously reported different susceptibilities of periodontopathic and nonperiodontopathic bacteria to antimicrobial peptides and phagocytosis by neutrophils. Differences between the two groups of bacteria may exist also in their ability to induce immune responses from the host. Therefore, we evaluated the effects of various oral bacteria on innate immune responses by gingival epithelial cells.

Material and Methods: HOK-16B cells were cocultured with live or lysed nonperiodontopathic (n = 3) and periodontopathic (n = 5) bacterial species. The levels of human beta defensin-1, -2 and -3, and of the cathelicidin, LL-37, were examined by real-time reverse transcription-polymerase chain reaction, and the accumulated interleukin-8 and interleukin-1α were measured by enzyme-linked immunosorbent assay.

Results: Nonperiodontopathic bacteria up-regulated some antimicrobial peptides without affecting the levels of cytokines. In the periodontopathic group, the orange-complex bacteria induced antimicrobial peptides and interleukin-8 efficiently, but the red-complex bacteria often demonstrated suppressive effects. In contrast to live bacteria, bacterial lysates had no suppressive effects. In addition, some bacterial lysates demonstrated a reduced ability to induce antimicrobial peptides compared with live bacteria.

Conclusion: The nonperiodontopathic, the orange-complex, and the red-complex bacteria had different effects on the innate immune responses from gingival epithelial cells, which may affect the outcome of their host–microbial interaction in gingival sulcus.
ISSN
0022-3484
Language
English
URI
https://hdl.handle.net/10371/69798
DOI
https://doi.org/10.1111/j.1600-0765.2007.00974.x
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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