S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Theses (Ph.D. / Sc.D._치의학과)
Effects of smoking on trace metal levels in saliva
흡연이 타액내 미량 금속 수준에 미치는 영향
- Issue Date
- 서울대학교 대학원
- Thesis(doctors) --서울대학교 대학원 :치의학과(구강내과·진단학전공),2010.2.
- The aim of this study was to compare the salivary levels of trace metals between nonsmokers
and smokers using inductively coupled plasma mass spectroscopy (ICP-MS).
The effect of pre-treatment methods on the accuracy of ICP-MS analysis and daily
variations in trace metal levels in saliva were also investigated.
The participants were 10 male non-smokers (mean age: 27.4 ± 3.4 years) and 30 male
smokers (mean age: 26.5 ± 4.1 years). Unstimulated whole saliva was collected.
Salivary flow rate, the number of metal restorations in the oral cavity, the level of blood
contamination in the saliva, and the levels of cotinine and trace metals in the saliva of
each participant were determined.
The obtained results were as follows:
1. Direct dilution of saliva samples with nitric acid showed the most accurate ICP-MS
results among the tested pre-treatments.
2. Trace metal levels in saliva showed wide daily variations.
3. Trace metal levels in saliva were not affected by the number of metal restorations in
the oral cavity.
4. The concentrations of magnesium, aluminum, and copper in saliva had significant
negative correlations with salivary flow rate.
5. The salivary concentrations and salivary secretion rates of cotinine and aluminum
were significantly increased in smokers compared with non-smokers.
In conclusion, saliva can be a medium for trace metal analysis. Smoking status has
significant effects on cotinine and aluminum levels in human saliva.
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