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Analysis of differential gene expression in auricular lymph nodes draining skin exposed to sensitizers and irritants
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ku, Hyun-Ok | - |
dc.contributor.author | Jeong, Sang-Hee | - |
dc.contributor.author | Kang, Hwan-Goo | - |
dc.contributor.author | Pyo, Hyun-Mi | - |
dc.contributor.author | Cho, Joon-Hyoung | - |
dc.contributor.author | Son, Seong-Wan | - |
dc.contributor.author | Ryu, Doug Young | - |
dc.date.accessioned | 2009-08-12T07:27:14Z | - |
dc.date.available | 2009-08-12T07:27:14Z | - |
dc.date.issued | 2007-12-16 | - |
dc.identifier.citation | Toxicol. Lett. 177, 1–9 | en |
dc.identifier.issn | 0378-4274 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7027 | - |
dc.description.abstract | There has been some concern that certain non-sensitizing irritants may yield false positive results in the murine local lymph node assay (LLNA). This study compared gene expression profiles in lymph nodes draining skin following exposure to sensitizers and irritants, to identify gene transcripts that could distinguish sensitizers from irritants. After treating CBA/N mouse ears for 3 days with the sensitizers 1-chloro-2,4-dinitrobenzene, 2-phenyl-4-ethoxymethylene-5-oxazolone, or toluene-2,4-diisocyanate or the non-sensitizing irritants croton oil or nonanoic acid, auricular lymph nodes and ear tissues were excised. Sensitizer-induced changes in parameters such as ear thickness, lymph node weight, and cell count also occurred in irritant-treated mouse tissues. However, gene transcripts such as Ifi27, Il12rb1, Ifng, and Zbp1, which are related to T-cell activation, were shown by gene expression microarrays and real-time RT-PCR analyses to be up-regulated in auricular lymph nodes by sensitizers exclusively. These findings suggest that gene expression analysis may enable distinction between sensitizing chemicals and non-sensitizing irritants. | en |
dc.language.iso | en | - |
dc.publisher | Elsevier | en |
dc.subject | Murine local lymph node assay | en |
dc.subject | Microarray | en |
dc.subject | Ifi27 | en |
dc.subject | Il12rb1 | en |
dc.subject | Ifng | en |
dc.subject | Zbp1 | en |
dc.title | Analysis of differential gene expression in auricular lymph nodes draining skin exposed to sensitizers and irritants | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 구현옥 | - |
dc.contributor.AlternativeAuthor | 정상희 | - |
dc.contributor.AlternativeAuthor | 강환구 | - |
dc.contributor.AlternativeAuthor | 표현미 | - |
dc.contributor.AlternativeAuthor | 조준형 | - |
dc.contributor.AlternativeAuthor | 손성완 | - |
dc.contributor.AlternativeAuthor | 류덕영 | - |
dc.identifier.doi | 10.1016/j.toxlet.2007.12.003 | - |
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