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Gateway RFP-fusion vectors for high-throughput functional analysis of genes

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dc.contributor.authorPark, Jae-Yong-
dc.contributor.authorHwang, Eun Mi-
dc.contributor.authorPark, Nammi-
dc.contributor.authorKim, Eunju-
dc.contributor.authorKim, Dong-Gyu-
dc.contributor.authorKang, Dawon-
dc.contributor.authorHan, Jaehee-
dc.contributor.authorChoi, Wan Sung-
dc.contributor.authorRyu, Pan Dong-
dc.contributor.authorHong, Seong-Geun-
dc.date.accessioned2009-08-12T07:40:20Z-
dc.date.available2009-08-12T07:40:20Z-
dc.date.issued2007-
dc.identifier.citationMol. Cells 23, 357-362en
dc.identifier.issn1016-8478-
dc.identifier.urihttp://molcells.inforang.com/about/journal_view.html?uid=257-
dc.identifier.urihttp://hdl.handle.net/10371/7031-
dc.description.abstractThere is an increasing demand for high throughput
(HTP) methods for gene analysis on a genome-wide
scale. However, the current repertoire of HTP detection
methodologies allows only a limited range of cellular
phenotypes to be studied. We have constructed two
HTP-optimized expression vectors generated from the
red fluorescent reporter protein (RFP) gene. These vectors
produce RFP-tagged target proteins in a multiple
expression system using gateway cloning technology
(GCT). The RFP tag was fused with the cloned genes,
thereby allowing us localize the expressed proteins in
mammalian cells. The effectiveness of the vectors was
evaluated using an HTP-screening system. Sixty representative
human C2 domains were tagged with RFP and
overexpressed in HiB5 neuronal progenitor cells, and
we studied in detail two C2 domains that promoted the
neuronal differentiation of HiB5 cells. Our results show
that the two vectors developed in this study are useful
for functional gene analysis using an HTP-screening
system on a genome-wide scale.
en
dc.description.sponsorshipWe appreciate the helpful advice of Dr. Tobias
Meyer and Dr. Won Do Heo of Stanford University in the
construction of the set of entry clones of human C2 domains. This
work was supported by a grant from the Basic Research Program
of the Korea Science and Engineering Foundation (R01-2002-
000-00128-0), and a Korea Research Foundation Grant (KRF-
2006-005-J04204).
en
dc.language.isoen-
dc.publisher한국분자·세포생물학회 = Korean Society of Molecular and Cellular Biologyen
dc.subjectGateway Cloning Systemen
dc.subjectRed Fluorescent Proteinen
dc.subjectHigh Throughput Screening Systemen
dc.titleGateway RFP-fusion vectors for high-throughput functional analysis of genesen
dc.typeArticleen
dc.contributor.AlternativeAuthor박재용-
dc.contributor.AlternativeAuthor황은미-
dc.contributor.AlternativeAuthor박남미-
dc.contributor.AlternativeAuthor김은주-
dc.contributor.AlternativeAuthor김동규-
dc.contributor.AlternativeAuthor강다원-
dc.contributor.AlternativeAuthor한재희-
dc.contributor.AlternativeAuthor최완성-
dc.contributor.AlternativeAuthor류판동-
dc.contributor.AlternativeAuthor홍성근-
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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