Publications

Detailed Information

Molecular differentiation of common promoters in Salmonella class 1 integrons

Cited 16 time in Web of Science Cited 17 time in Scopus
Authors

Kim, Tae-Eun; Kwon, Hyuk-Joon; Cho, Sun-Hee; Kim, Shukho; Lee, Bok-Kwon; Yoo, Han Sang; Park, Yong Ho; Kim, Sun-Joong

Issue Date
2006-11-22
Publisher
Elsevier
Citation
J. Microbiol. Methods 68, 453-457
Keywords
Class 1 integronNon-typhoidal SalmonellaAntibiotic-resistancePromoter
Abstract
The integron is a mobile gene element which harbors antibiotic-resistance gene cassettes capable of site-specific integration. Among the four known types of integrons, the class 1 integron has been associated with multidrug-resistance in pathogenic bacteria. These gene cassettes have been the focus of a series of studies. The gene cassettes share a common promoter, and their expression levels are affected not only by their proximity to the promoter, but also by the strength (weak, hybrid and strong) of the common promoter, P1, as well as the presence of the additional promoter, P2. In this study, we developed molecular methods for the differentiation of promoter structures using PCR, restriction enzyme analysis, and polyacrylamide gel electrophoresis, and have applied them to the characterization of class 1 integrons in 33 non-typhoidal Salmonella serotypes in Korea. Class 1 integrons were detected in four serotypes: S. Derby (SD), S. Istanbul (SI), S. Paratyphi B (SPB), and S. Livingstone (SL), and the amplicon sizes were 1.0 Kb (SD, SI and SPB) and 2.0 Kb (SL). All of the 1.0 kb amplicons harbored gene cassettes (aadA1 or aadA2), but the 2.0 kb amplicon harbored three (dhfrXII-orfF-aadA2) gene cassettes, which conferred streptomycin/spectinomycin (aadA) and trimethoprim (dhfr) resistances. Our promoter structure study revealed three types of promoters; strong P1 (SD), weak P1 (SPB and SL), and weak P1 + P2 (SI). In conclusion, the class 1 integrons were detected in Korean NTS, and their promoter structures were found to be variable. Therefore, our methods may prove helpful in terms of our understanding of molecular diversity, as well as the transmission of class 1 integrons and phenotype-genotype relationships in antibiotic-resistance.
ISSN
0167-7012
Language
English
URI
https://hdl.handle.net/10371/7050
DOI
https://doi.org/10.1016/j.mimet.2006.09.019
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share