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임신중 투여된 Azathioprine에 의한 신생 흰쥐 비장의 면역반응 억제에 관한 연구
Study on the Inhibition of the Immune Response of the Neonatal Rat Spleen by the Azathioprine Administered during Pregnancy

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Authors
장가용; 조사선; 이왕재
Issue Date
1983-09
Publisher
서울대학교 의과대학
Citation
Seoul J Med, Vol.24 No.4, pp. 353-362
Abstract
The following experiment was performed to study the inhibition mechanism of the immune response in
the neonatal rat spleen by azathioprine administered
during pregnancy.
The experimental animals were the two hundred
neonatal rats which were born by sixty normal Sqrague-
Dawley pregnant rats, of which 45 rats 8mgjkg
of azathioprine were administered to orally at the
7th gestational day.
Above two hundred experimental animals were
divided into 6 groups as follows:
Group 1: Neonatal rats to which T-dependent antigen,
Sheep Red Blood Cells (SRBC)
were injected intraperitoneally, born by
normal pregnant rats.
Group 2: Neonatal rats to which SRBC were injected
intraperitoneally, born by pregnant
rats to which azathioprine had already
been given orally during pregnancy.
Group 3: Neonatal rats to which SRBC were injected
intraperitoneally and thymus cells
derived from outbred neonatal rats were
injected intravenously, born by pregnant
rats to which azathioprine had already
been given orally during pregnancy.
Group 4: Neonatal rats to which T-independent
antigen, Dextran was injected intraperitoneally,
born by normal pregnant rats.
Group 5: Neonatal rats to which Dextran was
injected intraperitoneally, born by pregnant
rats to which azathioprine had already
been given orally during pregnancy.
Group 6: Neonatal rats to which Dextran was
injected intraperitoneally and thymus
cells derived from outbrcd neonatal rats
were injected intravenously, born by
pregnant rats to which azathioprine had
already been given orally during pregnancy.
Antigen was injected intraperitoneally to above
each group on the 1th, 8th and 12th weeks after
birth and then, the spleen was resected on the 3rd
and 7th days after injection of antigen for the plaque
assay and histochemical studies.
The following results were obtained:
1. Many plaque-forming cellsCPFC) were observed on the 3rd day after injection of antigen in all
groups to which SRBC were injected, while many
PFC were observed on the 7th day after injection
of antigen in all groups to which Dextran was
injected.
2. More PFC were observed in Group 1 than Group
2, and much more PFC were observed in Group
3.
3. There were no significant differences in number
of PFC between Dextran·Groups(Group 4, Group
5 and Group 6).
4. There was strong tendency to recover the immunoIogicaI
function with increasing age in SRBC·
Groups(Group 1, Group 2 and Group 3).
5. Above results were in good accordance with
those of histochemical observations.
6. It is strongly suggested that administration of
azathioprine during pregnancy did suppress the
development of fetal thymus, and that as a result
of that suppression peripheral Tvhelper cells were
depleted and consequently the formation of antibody
was inhibited due to the insufficient colla'
boration between T cells and B cells.
ISSN
0582-6802
Language
Korean
URI
https://hdl.handle.net/10371/7148
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 24 No.4 (1983)
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