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트롬보프라스틴의 보존법에 관한 연구 : Preservation of Reconstituted Thromboplastin of various Products

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서울대학교 의과대학
Seoul J Med, Vol.17 No.3, pp. 171-178
Thromboplastin is one of the most widely used biological
products in our country; and like most biolo·
gical products it is quite expensive and it is difficult
to obtain a proper commercial product of it. If it is
reconstituted with dist illed water it must he used
within hours and the remainder is discarded even
though the instruction of the make-r indicates that
they are stable for some days.
Moreover there is usually small number of daily requests
for prothromhin time tests in smalllaLoratories,
and considcrnlilc amount of reconstituted thromboplastin,
therefore. would be discarc!c'd.
It is quite apparent that large amount of recnnst itutcd
thromboplastin would become useless for this
reason unless otherwise treated to save its sensitivity
longer than several hours after reconstitution. In order
to find a suitable method of its storngc, which onablr-s
to maintain its sensitivity we performed a series of
r-xpr-rimeut s. sur'h as fre('zing, lyophilizing and several
First trial was to sec the cffrct of fre('zing store;
thn t is reconstituted remainder of th romboplasrins
were stored [or zen up to 1~ days and in the meantime
the prothrombin time tests were performed every day
with the [rozr-u-storcd sample.
Thromboplastins were thawed whenever we use it
at room temperature and evaluate the' scnsitivit y of
them both on the normal and almormal plasma.
Second trial was to observe the results when we
clevide them into several parts of aliquots in Kahn
tubes to keep them in refrigerator or deep freezer for
one through ten days, With these stored thromboplastin,
prothrombin time were checked every day to see the fluctuations in the thromboplastin sensitivity
if there is any.
When we were following this method, we were partieulary
interested in the way how to thaw the frozen
thromboplastin since varieties of thawing procedure
would hring about different results; for example, thawing
them at water bath or at room temperature may
perhaps results in different ways.
Third trial was to re-Iyophilize the reconstituted
thromboplastin. The reconstituted thromboplastin were
lyophilized nnce again and the changes in their sensitivity
were evaluated in accordance with prothrombin
Fourth trial was to evaluate the effects nf repeated
freezing and thawing on the stability of thromboplastins.
The results made from the this study arc as follows.
First, the remainders of daily reconstituted thromboplastin
left over being used is not adequate for prothrombin
time test even though stored in deep freezer.
Only 2 days of storage is acceptable as the maximum
of its allowance limit, if forced to use again.
Second, the best method for to store reconstituted
is that thromboplastin is divide into several aliquots
and storing in a deep freezer.
And the stability of thromboplastins was varia hlc
among the various products of thromboplast ins.
Third, the sensitivity of thromboplastin to standard
coagulation factor deficient plasmas are also variable among the products examined.
Fourth, rclyophilization of reconstituted thromboplastin,
is not acceptable for storage.
Fifth, the prohlem how to thaw the frozen thromboplastin
is insignificant and not affected whether the
thromboplastin is thawed in room temperature or in
water bath.
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