항암제 Incubation이 가토동종피부이식에 미치는 영향

Abstract

An appealing method to lower the risk of systemic 'Immunosuppression is local treatment of the allograft. 'Billingham and colleagues reported in 1951 the first "time that topically applied cortisone acetate doubled the survival of rabbit skin allografts. Woodruff and Llaurado confirmed these findings with local 9-afluoro- cortisol acetate in the same type of experiment. .Since then a variety of chemical agents, such as 'Triamcinolone Acetonide, Thalidomide. Urethan and -chemotherapeutic agents have been utilized to prolong -the allograft survival. However, in those studies. systemic dose of drugs .either adversely affected graft vascularization and "healing or were toxic or even lethal to the recipient. 'The author conducted analogous experiment using rabbit skin grafts incubated in 5-f1uorouracil. Endoxan and Cylocide to elucidate the effectiveness. The rabbit skin grafted by the technique described by Billingham using 20mm diameter. belly skin grafts from adult donors. These were incubated in 5-FU solution 50mg/cc, in Endoxan 40mg/cc. in Cylocide 40mg/cc respectively and in normal saline solution for control group for 60 minutes at room temperature and were rinsed in saline and then grafted immediately. There were considerable prolongation of allograft survival achieved by pretreatment with agents such as 5-FU, Endoxan and Cylocide compare to those of control group which was treated with only normal saline solution.