S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 17 No.4 (1976)
Studies on the Cellular Immunity and Enhancing Factors in MCA-induced Sarcoma in CsH/HeN Mice
MCA 유발육종에 있어서의 세포성면역과 증강요인에 관한 연구
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.17 No.4, pp. 369-382
- Colony inhibition assay in-vitro using the
peritoneal cells as the effector cells and in-vivo
transplantation method were adopted to demonstrate
cellular and humoral immunity to MCAinduced
sarcoma in tumor-bearing and in mice
immunized with various doses of MCA tumor
Transplantation of:;MCA sarcoma III normal
immune strain of tumor origin and III allogeneic
mice revealed that some imrnunolocgial surveillance
mechanism is operating normally.
Peritoneal cells from immune mice consistently
inhibited colony formation of plated target
tumor cells in-vitro, and the confrontation of
the peritoneal cells with tumor antigen or
sera from mice carrying advanced tumors,
before the treated peritoneal cells being added
to plated target tumor cells, abrogated the antitumor
activities of those cells.
Peritoneal cells from mice bearing advanced
tumors were found inactive in the inhibition of
colony formation in-vitro and although the sera
from the same mice enhanced dramatically the
tumor ~graft establishment in-vivo, it was destructive
on the plated tumor cells in-vitro.
The doses of killed tumor cells on which the
immunoprotection of the host against the same
viable tumor cells was dependent were investigated
with in-vivo challenge method and a narrow zone of tumor antigen dose for efficient
protection was found to exist in the 5-weeks'
schedule of immunization with one-week interval
and via I.P. injections.