Immunological Studies on the MeA-induced Sarcoma using the MIF Technique

Abstract

It has been generally agreed that in a che. mically induced, tumor the tumor specific antigen -could be identified by circulating humoral antibody and transplantation test among inbred ani. mals. To quantify the cellular immunity to the MCA- induced tumor and to assess the in vitro effect -of serum of mice which were bearing advanced tumors, the authors adopted the MIF test. And the results obtained are summarized as follows: 1. MIF technique has been established using the mammalian tumor cells as antigen with the ratio of peritoneal cells to tumor cells as 10: 1 or 1: 1, and the test could be performed with good results by adding the tumor cells directly into the media of the chamber instead of mixing with the peritoneal cells in the capillary tubes. :2. The antigenicity of the MCA-induced sareorna in C3H/HeN mice was detected by MIF technique. When peritoneal cells obtained from mice sensitized with frozen-thawed tumor cells were challenged with the same tumor cells in vitro, they were inhibited significantly from migration on the glass surface as compared with those from normal mice. 3. In the host which had received viable tumor cells but not yet developed a grossly visible or palpable tumor, in vitro cell mediated immune response to the tumor cells(detected by MIF test) was the same as that of actively sensitized host. 4. The blocking effect on the cell mediated immunity in vitro of the sera obtained from mice which were bearing advanced tumors was detected by using the sera as substitute for normal sera in the media of MIF chambers and in the procedure of washing the peritoneal cells before contact with the tumor cells.