S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Epidermal cellular response to poly(vinyl alcohol) nanofibers containing silver nanoparticles
- Chun, Ja Young; Kang, Hyun Ki; Jeong, Lim; Kang, Yun Ok; Oh, Ju-Eun; Yeo, In-Sung; Jung, Sung Youn; Park, Won Ho; Min, Byung-Moo
- Issue Date
- Colloids and Surfaces B: Biointerfaces, 78 (2010) 334–342
- Poly(vinyl alcohol); Nanofibers; Silver; Nanoparticles; Ions; Epidermal cells; Cytotoxicity
- Aheat-treatedPVAnanofibrous matrix containing silver (Ag)wasprepared by electrospinning an aqueous
10 wt% PVA solution and followed by heat treatment at 150 ◦C for 10 min. The average diameter of the asspun
and heat-treated PVA nanofibers was 330 nm. The heat-treated PVA nanofibrous matrix containing
Ag was irradiated with UV light to transform the Ag ions in the nanofibrous matrix into Ag nanoparticles.
The in vitro cytotoxicity of the Ag ions and/or nanoparticles on normal human epidermal keratinocytes
(NHEK) and fibroblasts (NHEF) cultures was examined. ThePVAnanofibrous matrix containing Ag showed
slightly higher level of attachment and spreading in the early stage culture (1 h) than the PVA nanofibers
without Ag (control). However, compared with the PVA nanofibers without Ag, the heat-treated and UVirradiated
PVA nanofibers, containing mainly Ag ions and nanoparticles, respectively, showed reduced
cell attachment and spreading. This shows that both Ag ions and Ag nanoparticles are cytotoxic to NHEK
and NHEF. There was no significant difference in cytotoxicity to NHEK and NHEF between Ag ions and
Ag nanoparticles. NHEF appeared to be more sensitive to Ag ions or particles than NHEK. In addition, the
residual nitrate ions (NO3−) in the PVA nanofibers had an adverse effect on the culture of both cells.
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