d-Amphetamine이 Rat의 전뇌 5-hydroxytryptamine(5-HT), 5-hydroxyindole 3-acetic acid(5-HIAA) 함량 및 Monoamine Oxidase(MAO)활성도에 미치는 영향
The Effect of d-amphetamine on the content of 5-hydroxytryptamine, 5-hydroxyindole 3-acetic acid and the activity of monoamine oxidase of rat brain
- 서유헌; 홍사악; 박찬웅
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.20 No.3, pp. 289-296
- Amphetamine causes a variety of CNS stimulating
effects and various behavioral changes in men and
animals, which were first described by Piness (1930)
and Alles et al. (1933). It is well known that amphetamine
induces psychosis closely mimicking paran<
lid schizophrenia, and has been used as a research
tool for the etiologic role of Schizophrenia.
Many investigators have generally believed that
.amphetarnine was a prototype activator of catecholamine
mechanisms in the CNS, but more recent findings
strongly suggest that 5-hydroxytryptamine(5HT)
is involved in, at least, some of the effects of d.
Also, d-arnphetamine has been known to be a
reversible inhibitor of monoamine oxidase (MAO)
'with a preference for MAO type A in vitro.
This present study was set up to determine if, and
'to what extent, the turnover of 5-HT is influenced
>by d-amphetamine administration.
5-HT and its major metabolite, 5-hydroxyindole.::
J-acetic acid (5-HIAA) were determined fluorometri-
cally at various times after the intraperitioneal inje.
ction of d-amphetamine (2mg/kg) by the combined
method of Curzon and Green (1970), and Shellenberger
et al. ('71). Monoamine oxidase activity of
whole brain towards the substrate kynuriamine was
.measured by the modified method of Kraml('65). Following results were obtained:
1. 5-HT level showed a maximal increase (126. 6%
of control) at 15 min post-injection and increase
lasted for 30 min, and thereafter 5-HT decreased till
24 hr post- injection, and returned to nearly control
level at 48 hr after injection.
2. 5-HIAA level began to decrease at 30 min postinjection,
reaching lowest levels at approximately the
1 hr-4 hr post-iniection (78% of control), and returned
to control level at 24 hr after injection, and
thereafter the level increased to HI. 7% of control
at 48 hr post-injection.
3. MAO activity showed the lowest level at 15
min post-iniection (86. 9% of control), and thereafter
slightly low activity persisted (90. 3%~97. 1% of
control), till 2 hr post-injection, MAO activity began
to increase at 4 hr. post-injection, reaching the
highest level at 24 hr post-injection (142.7% of
4. 5-HIAA/5-HT ratio began to decrease at 15
min post-injection, and decrease lasted for 2 hrs.
Thereafter, the ratio showed to increase, reaching
the highest value at 24 hr after injection (141.1%
We strongly suggest that 5-hydroxytryptamine
turnover is influenced by d-amphetamine administration
through the effect on the activity of monoamine