S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 15 No.2 (1974)
에르릿히 복수암조직에 있어서 젖산의 유리 아미노산 전환에 관한 연구
On Cytoplasmic Free Amino Acids Converted from Exogenous Lactate in Vitro Ehrlich Ascites Tumor Tissue in Mice
|dc.identifier.citation||Seoul J Med, Vol.15 No.2, pp. 129-136||-|
|dc.description.abstract||"The conversion of amino acids from L-Iactate-UCa
was investigated in the normal liver. and cancer
tissues which obtained from the tumor-bearing
animals (Ehrlich ascites tumor). Tissue homogenates
of tumors and normal liver were incubated for 10
minutes in the water bath at the constant temperature
The assay of amino acid was performed quantitatively
and qualitatively by the method of thin
layer chromatography. in which two dimensional
ascending method was used. The amino acid spots
on the chromatoplate were identified by means of
Rf value and colour developed in the ninhydrin
.spray. The density and radioactivity of each spot
were measured simultaneously by use of the Radio
Thin-Layer Chromatogram Scanner.
The specific activity (cprn/uM) of each amino
.acid was obtained from the result of activity (cpm/
gm wet tissue) divided by amount (uM/gm wet
tissue). The conversion ratio of each converted
amino acid(%) was calculated as the ratio of S. A.
of converted amino acid (cpm/uM) and S. A. of
lactate added (225XI0' cpm/uM).
The data obtained. were summerized as follows;
1. The amino acids converted from the added Llactate-
U-C" were alanine. serine. glycine. glutamic
.acid and aspartic acid in the cancer tissues and also
in the normal hepatic tissues.
2. The specific activity of the converted amino
acids were alanine 2245. serine 2353. glycine 9755
(highest). glutamic acid 2703. and aspartic acid
1503 cpm/uM. respectively. in the cancer tissues.
3. The conversion ratio from the added L-IactateU-
C" of each converted amino acid was alanine
1.00. serine 1. 05. glycine 4.34. glutamic acid 1.20 and aspartic acidO.67%. Total conversion ratio was
8.26% in the cancer tissues.
4. In the normal hepatic tissues. the specific
activity of each converted amino acid was alanine
2702. serine 2900. glycine 3721. glutamic acid 9556.
(highest) and aspartic acid 1561 cpm/uM. respectively.
5. The conversion ratio calculated from the S. A.
of the converted amino acid and the S. A. of the
added lactate was alanine 1. 20. serine 1. 29. glycine
1.65, glutamic acid 4.25. and aspartic acid 0.69%.
respectively. Thus the total conversion ratio was
9.08% in the normal hepatic tissues.
From the above results. it was concluded that
the accumulated lactic acid in the cancer tissues
was not only used for further metabolites in carbohydrate
metabo ism. but also considerable fraction
of it was converted into the various amino acids
which were necessary in the cancer tissue proliferation.
|dc.title||에르릿히 복수암조직에 있어서 젖산의 유리 아미노산 전환에 관한 연구||-|
|dc.title.alternative||On Cytoplasmic Free Amino Acids Converted from Exogenous Lactate in Vitro Ehrlich Ascites Tumor Tissue in Mice||-|
|dc.contributor.AlternativeAuthor||Kong, Jin Kook||-|
|dc.citation.journaltitle||서울 의대 잡지||-|
|dc.citation.journaltitle||서울 의대 학술지||-|
|dc.citation.journaltitle||Seoul Journal of Medicine||-|
- Appears in Collections:
- College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 15 No.2 (1974)