Detection of Human Papillomavirus DNA in Condylomata Acuminata by In Situ Hybridization

Abstract

In situ hybridization using biotinylated HPV(Human papillomaviruses) probes was tried in order to detect the presence of HPV DNA in 17 patients with condyloma acuminatum. Fourteen cases responded with positive hybridization signals to HPV 6/11. The specific signals were seen focally in the nuclei of superficial epithelial cells. None of the cases were positive for HPV 16/18. There was no cross hybridization between HPV 6/11 and HPV 16/18 under the stringent conditions used in these experiments. So it could be said that HPV 6/11-related sequences were found in usual condyloma acuminatum.