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Expression of Osteocalcin and Transglutaminase and Labelling of Bromodeoxyuridine during Fracture Healing in the Rat Tibia

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Authors
Kim, Woo Ho; Seong, Sang Cheol; Lee, Myung Chul; Song, Kye Yong; Park, Sang Chul
Issue Date
1990-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med, Vol.35 No.1, pp. 9-18
Keywords
TransglutaminaseOsteocalcinBromodeoxyuridineFracture healing
Abstract
The expression of osteocalcin and transglutaminase C(TGase C) during fracture
healing was inwstigated with immunohistochemical studies. A transverse osteotomy
was made at the proximal tibia in Sprague-Dawley male rats and immobilized with a
small external skeletal fixator. The animals lU!l'e sacrificed serially I, 3, 5, 7, 14, 42 days
respectively after fracture. Longitudinal sections of the healing bone were stained with
pohclonal antibody against osteocalcin and TGase C, and monoclonal antibody against
bromodeoxyuridine. During the intramembranous bone formation at the periosteum around
the fracture site, osteocalcin was strongly expressed in the proliferating osteoprogenitor
cells from the 1st day of fracture, and then, in osteoblasts, osteoid matrix and osteocytes.
The expression of TGase C was weakly positive in both osteoprogenitor cells and
osteoblasts. Ai the site of endochondral bone formation, which was first reoealed 5 days
after fracture, cell proliferation occurred at the periphery of cartilaginous callus where the
number of cells stained with BrdU was highest During the maturation of callus, those cells
uere entrapped in the chondroid matrix and became larger and larger. Osteocalcin was
demonstrated in the cytoplasm of chondrocytes, while chondroid matrix was negatiwly
stained. TGase C was found in the cytoplasm of more centrally located and matured
chondrocytes as compared with osteocalcin. Osteoid matrix was stained with osteocalcin
but not with TGase C. These finding may suggest that osteocalcin participates in the early
phase of endochondral bone formation, while TGase C participates in the late phase,
suggesting the role of TGase C in matrix stabilization. But the reason for the difference in
the expression of TGase C between the endochondral bone formation and
intramembranous bone formation should be further inwstigated. Healing of IAA2Il
immobilized fracture in this study was predominantly induced by intramembranous ossification
rather than endochondral ossification. Periosteal osteoprogenitor cells appeared to
initiate and to lead bone formation after osteotomy. These findings indicate that preservation
of the periosteum is essential to achieve successful fracture healing.
ISSN
0582-6802
Language
English
URI
https://hdl.handle.net/10371/7327
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 35 No.1 (1994)
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