S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 35 No.2 (1994)
Effects of Acute Hyperoxia on Hyaluronic Acid and Histopathology of the Lung in Neonatal Ratst
- Kim, Beyong Il; Choi, Jung-Hwan; Yun, Chong Ku; Chi, Je G.
- Issue Date
- Seoul J Med, Vol.35 No.2, pp. 91-98
- We studied the effects of hyperoxia()95% oxygen for 10 days) on changes
in body weight, changes in wet to dry lung weight ratio, changes in hyaluronic acid of
the lung, and morphologic changes in lung tissue compared with those of normoxia
(room air for 10 days) in Sprague-Dawley neonatal rat pups. In this acute hyperoxic
neonatal rat model, we tried to analyze the relationship of relative water content of the
wet lung and the amount of hyaluronic acid and morphologic change in lung tissue.
The results were as follows: 1) Increase of postnatal body weight among survivors was
stunted significantly under hyperoxia compared with normoxia during 10 days-experiment.
2) No appreciable difference of wet to dry lung weight ratio was noted on the beginning
and the tenth day of experiment in neonatal rat pups between normoxia and
hyperoxia, but considerable increased wet to dry lung weight ratio was noted significantly
at 5, 7 days of hyperoxia suggesting that the relative water content of the wet
lung was increased on the fifth, seventh day of experiment in hyperoxia compared with
normoxia, 3) The amount of hyaluronic acid per wet lung decreased sequentially according
to increase of postnatal age in normoxia, but a considerably increased amount
of hyaluronic acid per wet lung was noted significantly on the fifth, the seventh day of
experiment in hyperoxia. The difference in amount of hyaluronic acid per wet lung was
not significant on the tenth day of experiment between normoxia and hyperoxia. These
results suggested that the changing pattern of amount of hyaluronic acid per wet lung
coincided with that of the relative water content of the wet lung in hyperoxia. 4) Pulmonary
edema, interstitial inflammatory cell hypercellularity, and localization of
hyaluronic acid in interstitial lung lesion were observed by light microscope at 7 days
of exposure in hyperoxia compared with normoxia. These results suggest the possible
role of hyaluronic acid on increase of water content and interstitial inflammatory cells
in acute lung injury due to hyperoxia in an experimental neonatal rat model.