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Clinical Significance of CD99 Down-Regulation in Gastric Adenocarcinoma

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dc.contributor.authorLee, Jung Hyun-
dc.contributor.authorKim, Seok-Hyung-
dc.contributor.authorLi-Hui Wang-
dc.contributor.authorChoi, Yoon-La-
dc.contributor.authorKim, Young Chul-
dc.contributor.authorKim, Jin Hee-
dc.contributor.authorPark, Tae Sung-
dc.contributor.authorHong, Yun-Chul-
dc.contributor.authorShin, Young Kee-
dc.date.accessioned2011-06-14T09:45:44Z-
dc.date.available2011-06-14T09:45:44Z-
dc.date.issued2007-05-
dc.identifier.citationClin Cancer Res; Vol.13(9); pp.2584-2591en
dc.identifier.issn1557-3265-
dc.identifier.issn1078-0432-
dc.identifier.urihttps://hdl.handle.net/10371/73425-
dc.description.abstractPurpose: CD99 is a cell adhesion molecule associated with human tumors. The aim of the present study was to characterize its role in the development and progression of human gastric adenocarcinoma.
Experimental Design: The expression of CD99 was investigated in 283 gastric adenocarcinomas and related lesions and 9 gastric carcinoma cell lines. We also analyzed the methylation status of CD99 gene by using methylation-specific PCR and examined loss of heterozygosity (LOH) of this gene locus by using an intragenic marker. Moreover, we assessed whether SP1, a positive transcription factor for CD99, is expressed in these samples.
Results: We found that the decreased expression of CD99 was strongly associated with poor survival and unfavorable clinicopathologic variables. Promoter region methylation (15 of 89, 16.9%) and LOH (21 of 74, 28.4%) were observed and significantly associated with CD99 down-regulation (P < 0.05). In addition, most of the gastric adenocarcinoma cases with CD99 down-regulation had reduced expression of SP1 (47 of 103, 45.6%; P < 0.01). This relationship between CD99 and SP1 was consolidated by using SP1 small interfering RNA transfection experiment and CD99 promoter luciferase assay. Furthermore, we showed that CD99 down-regulation was associated with proliferation and migration in gastric carcinoma cell line.
Conclusion: These observations suggest that CD99 down-regulation is a critical event in the progression of gastric adenocarcinoma, and CD99 promoter methylation, CD99 LOH, and SP1 down-regulation were responsible for the down-regulation of CD99.
en
dc.language.isoenen
dc.publisherAmerican Association for Cancer Researchen
dc.titleClinical Significance of CD99 Down-Regulation in Gastric Adenocarcinomaen
dc.typeArticleen
dc.contributor.AlternativeAuthor이정현-
dc.contributor.AlternativeAuthor김석형-
dc.contributor.AlternativeAuthor최윤라-
dc.contributor.AlternativeAuthor김영철-
dc.contributor.AlternativeAuthor김진희-
dc.contributor.AlternativeAuthor박태성-
dc.contributor.AlternativeAuthor홍윤철-
dc.contributor.AlternativeAuthor신영기-
dc.identifier.doi10.1158/1078-0432.CCR-06-1785-
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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