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NMR and Circular Dichroism Studies on Human CD99 Transmembrane Domain

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Authors
Kim, Hai-Young; Shin, Joon; Shin, Young-Kee; Park, Seong-Hoe; Lee, Weontae
Issue Date
2003
Publisher
한국자기공명학회
Citation
J Kor magn reson soc 7(1):37-45(2003)
Keywords
CD99transmembrane domainCircular dichroismNMR
Abstract
Human CD99 is a ubiquitous 32-kDa transmembrane protein encoded by mic2 gene. Recently it has been reported that expression of a splice variant of CD99 transmembrane protein (Type I and Type II) increases invasive ability of human breast cancer cells. To understand structural basis for cellular functions of CD99 Type II, we have initiated studies on hCD99TMcytoI/ using circular dichroism (CD) and multi-dimensional NMR spectroscopy. CD spectrum of hCD99TMytoI/ in the presence of 200mM DPC and CHAPS displayed an existence -helical conformation, showing that it could form an -helix under membrane environments. In addition, we have found that the cytoplasmic domain of CD99 would form symmetric dimmer in the presence of transmembrane domain. Although it has been rarely figured out the correlation between structure and functional mechanism of hCD99TMcytoI/, the dimerization or oligomerization would play an important role in its biological function.
ISSN
1226-6531
Language
English
URI
https://hdl.handle.net/10371/73481
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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