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An Improved Protocol of Biotinylated Tyramine-based Immunohistochemistry Minimizing Nonspecific Background Staining

Cited 24 time in Web of Science Cited 27 time in Scopus
Authors
Kim, Seok Hyung; Shin, Young Kee; Lee, Kyung Mee; Lee, Jung Sun; Yun, Ji Hye; Lee, Sun Myung
Issue Date
2003-01
Publisher
Histochemical Society
Citation
J Histochem Cytochem; Vol.51, No.1; pp.129–132
Keywords
immunohistochemistrytyraminebackground staining
Abstract
An immunohistochemical method using biotinyl tyramine was recently introduced
to amplify weak staining signals. Despite its high sensitivity, however, tyraminebased
immunostaining has been limited by its increased background staining. In this study,
to develop an improved protocol of biotinyl tyramine-based immunohistochemistry minimizing
the background staining, we determined which staining steps lead to the nonspecific
reaction and the most appropriate blocking agents for background-provoking steps.
Trypton casein peptone and distilled water with Tween-20 were shown to be most effective
as a blocking agent and a rinsing solution, respectively. In conclusion, we developed an
optimized protocol for biotinyl tyramine-based immunohistochemistry with minimal background staining.
ISSN
1551-5044(electronic)
0222-1554(print)
Language
English
URI
http://hdl.handle.net/10371/73496
DOI
https://doi.org/10.1177/002215540305100116
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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