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Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries
DC Field | Value | Language |
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dc.contributor.author | Chung, Junho | - |
dc.contributor.author | Park, Sojung | - |
dc.contributor.author | Kim, Dongjo | - |
dc.contributor.author | Rhim, Junghyo | - |
dc.contributor.author | Kim, Ikjung | - |
dc.contributor.author | Choi, Inhak | - |
dc.contributor.author | Yi, Kyesook | - |
dc.contributor.author | Ryu, Sungho | - |
dc.contributor.author | Suh, Pannghill | - |
dc.contributor.author | Chung, Doohyun | - |
dc.contributor.author | Bae, Youngmee | - |
dc.contributor.author | Shin, Youngkee | - |
dc.contributor.author | Park, Sunghoe | - |
dc.date.accessioned | 2011-07-22T05:39:01Z | - |
dc.date.available | 2011-07-22T05:39:01Z | - |
dc.date.issued | 2002-12 | - |
dc.identifier.citation | J Cancer Res Clin Oncol; Vol.128, No.12; pp.641–649 | en |
dc.identifier.issn | 0171-5216 | - |
dc.identifier.uri | https://hdl.handle.net/10371/73499 | - |
dc.description.abstract | PURPOSE: In the present study an antigen-mimetic peptide of the anti-JL1 leukemia-specific monoclonal antibody (mAb) was identified and characterized.
METHODS: From combinatorial peptide phage display libraries displaying the random linear heptapeptides and dodecapeptides, we selected clones with affinity to anti-JL1 mAb through repeated rounds of panning on a mAb-coated ELISA plate. The antigenicity and immunogenicity of the peptide epitopes were then studied using chemically synthesized peptides. RESULTS: The selected clones had the LXPSIP consensus sequence. Two synthetic peptides LPPSIPFGLTVGGGGS and LLPSIPNQAYLGGGGS specifically reacted with anti-JL1 mAb in ELISA. These two peptides were found to inhibit the interaction between anti-JL1 mAb and JL1 antigen-positive Molt-4 cells. Although the immune sera raised against the keyhole limpet hemocyanin-conjugated peptides failed to react with Molt-4 cells, it showed strong reactivity to the peptide epitope. However, one mAb raised by peptide immunization successfully bound to Molt-4 cells. CONCLUSION: An epitope-mimetic peptide of anti-JL1 mAb was found using combinatorial peptide phage display libraries. It induced strong humoral response against itself, but only a limited fraction of this humoral response was cross-reactive with the original JL1 antigen. | en |
dc.language.iso | en | en |
dc.publisher | Springer | en |
dc.subject | Phage display | en |
dc.subject | Combinatorial peptide library | en |
dc.subject | Mimotope-Monoclonal antibody | en |
dc.subject | JL1 antigen | en |
dc.title | Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정준호 | - |
dc.contributor.AlternativeAuthor | 박소정 | - |
dc.contributor.AlternativeAuthor | 김동조 | - |
dc.contributor.AlternativeAuthor | 임정효 | - |
dc.contributor.AlternativeAuthor | 김익정 | - |
dc.contributor.AlternativeAuthor | 최인학 | - |
dc.contributor.AlternativeAuthor | 이계숙 | - |
dc.contributor.AlternativeAuthor | 류성호 | - |
dc.contributor.AlternativeAuthor | 서방길 | - |
dc.contributor.AlternativeAuthor | 정두현 | - |
dc.contributor.AlternativeAuthor | 배영미 | - |
dc.contributor.AlternativeAuthor | 신영기 | - |
dc.contributor.AlternativeAuthor | 박성회 | - |
dc.identifier.doi | 10.1007/s00432-002-0390-x | - |
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