S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 35 No.4 (1994)
Genotyping via Amplification and Hhal Cleavage of Apolipoprotein E (ApoE) Gene in Patients with Coronary Artery Disease
- Issue Date
- Seoul J Med, Vol.35 No.4, pp. 265-273
- DNA amplification and HhaI cleavage for the apolipoprotein E (apofi)
genotyping is identified in 108 patients with coronary artery disease (CAD). A group
of randomly selected 100 normal individuals analysed as control samples. There
were no significant differences in apoE genotype frequencies between the two groups.
Analysis of variances was performed to test for mean differences in plasma lipids.
lipoprotein and apolipoprotein B (apoB) levels. Compared to controls, patients with
CAD had significantly higher levels of total cholesterol (TC) and low density
lipoprotein-cholesterol (LDL-ChoI). The ratios of LDL-Chol to apoB were also
elevated in the CAD group. The levels of TC and LDL-Chol in the E3j3 genotype of
patients were significantly higher than those of the controls. The high density
Iipoprotein-cholesteroI(HDL-ChoI) levels in E4j3 type of the patients were
significantly higher than those of E3j3 subjects. As phenotype analysis for the apoE
polymorphism differs somewhat among populations, so we discussed possible
reasons for these discrepancies.