정상 및 부신 적출 생쥐의 사구방 과립세포의 분비과립에 관한 형태학적 연구

Abstract

The juxtaglomerular granular cells of the mice were investigated by electron microscopy and light microscopy with the aim elucidating the morphological characteristics of the granules and the juxtaglomerular granular index (JGI) and granular cell index (Gel) were calculated under the various experimental conditions. They were normal control and one, two. and four week-survived animals after adrenalectomy. Some of the each group of the animals were injected cobalt nitrate subcutaneously. and sacrificed at thirty. sixty. and one hundred and twenty minutes after the injection. For the ultrastructural study, under the anesthesia of Nembutal the kidney was fixed by perfusion method with 2.3% glutaraldehyde in 45mM cacodylate buffer following prerinsing with heparinated 0.1% Ringer-Iidocaine solution. After the removal of the tissue, it was postfixed in O. 1% osmium tetroxide in 45mM cacodylate buffer by immersion. For the histological study the tissue was fixed in Zenker-formol solution, and stained by Wilson's method. The results observed were as follows: I. Large and round or oval granules. calssified as fully matured type III granules, were found in the cytoplasm of the juxtaglomerular granular cells in both the control and experimental groups. 2. Amorphous large granules that mean the coalescence of the granules were usually found in two and four week-survived animals after bilateral adrenalectomy. 3. One of the juxtaglomerular cells in adrenalectomized animal showed markedly distended rough surfaced endoplasmic reticulum. 4. Both of the granular cell and juxtaglomerular granular indices were iecreased after adrenalectomy, and the injection of cobalt nitrate decreased them. 5. The frequently appearing cell type was type 1 in normal control. In adrenalectomized condition. however. type 3-cells appeared more frequently. By the injection of cobalt nitrate to adrenalectomized animals type 3-cells were replaced by type I-cells.