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Nanowire-Based Delivery of Escherichia coli O157 Shiga Toxin 1 A Subunit into Human and Bovine Cells
DC Field | Value | Language |
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dc.contributor.author | Kwon, Nam Hoon | - |
dc.contributor.author | Beaux II, Miles F. | - |
dc.contributor.author | Ebert, Chad | - |
dc.contributor.author | Wang, Lidong | - |
dc.contributor.author | Lassiter, Brian E. | - |
dc.contributor.author | Park, Yong Ho | - |
dc.contributor.author | McIlroy, David N. | - |
dc.contributor.author | Hovde, Carolyn J. | - |
dc.contributor.author | Bohach, Gregory A. | - |
dc.date.accessioned | 2009-08-20T09:38:52Z | - |
dc.date.available | 2009-08-20T09:38:52Z | - |
dc.date.issued | 2007-07-27 | - |
dc.identifier.citation | Nano Lett. 2007, 7, 2718-2723 | en |
dc.identifier.issn | 1530-6984 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7392 | - |
dc.description.abstract | Silica nanowires (NWs) were used to introduce the Shiga toxin type 1 A subunit (StxA1) into cultured bovine and human epithelial cells. We
extended technology developed in our laboratories that employs fibronectin (Fn) to induce integrin-mediated uptake of NWs by coating NWs with StxA1 and Fn. The bonding strengths of Fn and StxA1 to the surface of NWs were measured by X-ray photoelectron spectroscopy. This technique demonstrated complex interactions between Fn, StxA1, and the NWs. Neutral red cytotoxicity assays and field emission scanning electron microscopy confirmed that the NW-StxA1-Fn complexes were effectively internalized and caused cell death. This indicates that NWs can carry StxA1 and potentially other toxic or therapeutic agents into eukaryotic cells. Ongoing studies include improved functionalizing of NWs aimed at increasing internalization efficiency and substituting ligands for specific cell targeting. | en |
dc.description.sponsorship | This work was supported, in part, by
the Idaho Agricultural Experiment Station, the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, Grant Number 04-04562 (C.J.H.), and Public Health Service grants, U54-AI-57141, P20-RR016454, and P20-RR15587 (G.A.B. and C.J.H.), and the University of Idaho-BANTech Initiative. This work was also supported by the W.M. Keck Foundation (D.N.M.) and the NSF, Grant Number EPS0132626 (D.N.M.). The authors thank Haiqing Sheng and Jie Li for the purification of recombinant StxA1. | en |
dc.language.iso | en | - |
dc.publisher | American Chemical Society | en |
dc.title | Nanowire-Based Delivery of Escherichia coli O157 Shiga Toxin 1 A Subunit into Human and Bovine Cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 권남훈 | - |
dc.contributor.AlternativeAuthor | 박용호 | - |
dc.identifier.doi | 10.1021/nl071179f | - |
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