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MG-63 세포주에서 Vascular Endothelial Growth Factor (VEGF) mRNA 발현에 대한 Insulin-like Growth Factor I (IGF-I)의 효과에 대한 연구 : The effects of Insulin-like Growth Factor I (IGF-I) on expression of Vascular Endothelial Growth Factor(VEGF) mRNA in MG-63 osteoblastlike cells
DC Field | Value | Language |
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dc.contributor.author | 서제덕 | - |
dc.contributor.author | 명훈 | - |
dc.contributor.author | 강나라 | - |
dc.contributor.author | 정필훈 | - |
dc.date.accessioned | 2011-10-12T01:28:17Z | - |
dc.date.available | 2011-10-12T01:28:17Z | - |
dc.date.issued | 2005-10 | - |
dc.identifier.citation | J Kor Oral Maxillofac Surg 2005;31:363-369 | en |
dc.identifier.issn | 1225-1585 | - |
dc.identifier.uri | https://hdl.handle.net/10371/74018 | - |
dc.description.abstract | Purpose: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression
in MG-63 cells and then to find the mechanism b which this regulation occurs. Materials and methods: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea (76.1 ㎍/㎖), actinomycin D (2.5 ㎍/㎖), cycloheximide (10 ㎍/㎖) were added 1 hour after treatment of 10 nM IGF-I. Results: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time- and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. Conclusion: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling. | en |
dc.language.iso | ko | en |
dc.publisher | 대한구강악안면외과학회 | en |
dc.subject | Osteogenesis | en |
dc.subject | Angiogenesis | en |
dc.subject | Vascular endothelial growth factor | en |
dc.subject | Insulin-like growth factor I | en |
dc.subject | MG-63 cell line | en |
dc.title | MG-63 세포주에서 Vascular Endothelial Growth Factor (VEGF) mRNA 발현에 대한 Insulin-like Growth Factor I (IGF-I)의 효과에 대한 연구 | en |
dc.title.alternative | The effects of Insulin-like Growth Factor I (IGF-I) on expression of Vascular Endothelial Growth Factor(VEGF) mRNA in MG-63 osteoblastlike cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | Suh, Je-Duck | - |
dc.contributor.AlternativeAuthor | Myung, Hoon | - |
dc.contributor.AlternativeAuthor | Kang, Nara | - |
dc.contributor.AlternativeAuthor | Choung, Pill-Hoon | - |
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