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Bradykinin-induced Ca2+ signaling in human oral squamous cell carcinoma HSC-3 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sohn, Byung Jin | - |
dc.contributor.author | Kang, Ji-Ah | - |
dc.contributor.author | Jo, Su-Hyun | - |
dc.contributor.author | Choi, Se-Young | - |
dc.date.accessioned | 2011-10-12T05:09:26Z | - |
dc.date.available | 2011-10-12T05:09:26Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | International Journal of Oral Biology 2009;34:73-79 | en |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://hdl.handle.net/10371/74036 | - |
dc.description.abstract | Cytosolic Ca
2+ is an important regulator of tumor cell proliferation and metastasis. Recently, the strategy of blocking receptors and channels specific to certain cancer cell types has emerged as a potentially viable future treatment. Oral squamous cell carcinoma is an aggressive form of cancer with a high metastasis rate but the receptormechanisms involved in Ca 2+ signaling in these tumors have not yet been elucidated. In our present study, we report that bradykinin induces Ca 2+ signaling and its modulation in the human oral squamous carcinoma cell line, HSC-3. Bradykinin was found to increase the cytosolic Ca 2+ levels in a concentration-dependent manner. This increase was inhibited by pretreatment with the phospholipase C-β inhibitor, U73122, and also by 2-aminoethoxydiphenyl borate, an inhibitor of the inositol 1,4,5-trisphosphate receptor. Pretreatment with extracellular ATP also inhibited the peak bradykinin-induced Ca 2+ rise. In contrast, the ATP-induced rise in cytosolic Ca 2+ was not affected by pretreatment with bradykinin. Pretreatment of the cells with either forskolin or phorbol 12-myristate 13-acetate (activators of adenylyl cyclase and protein kinase C, respectively) prior to bradykinin application accelerated the recovery of cytosolic Ca 2+ to baseline levels. These data suggest that bradykinin receptors are functional in Ca 2+ signaling in HSC-3 cells and may therefore represent a future target in treatment strategies for human oral squamous cell carcinoma. | en |
dc.description.sponsorship | This work was supported by the Korea Science
& Engineering Foundation grant (R13-2008-008-01001-0) through the Oromaxillofacial Dysfunction Research Center for the Elderly (ODRCE) at Seoul National University. | en |
dc.language.iso | en | en |
dc.publisher | The Korean Academy of Oral Biology | en |
dc.subject | Bradykinin | en |
dc.subject | oral squamous cell carcinoma | en |
dc.subject | HSC-3 cells | en |
dc.subject | phospholipase C | en |
dc.title | Bradykinin-induced Ca2+ signaling in human oral squamous cell carcinoma HSC-3 cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 손병진 | - |
dc.contributor.AlternativeAuthor | 강지아 | - |
dc.contributor.AlternativeAuthor | 조수현 | - |
dc.contributor.AlternativeAuthor | 최세영 | - |
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