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PDGF-B gene therapy accelerates bone engineering and oral implant osseointegration

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Issue Date
2010-01
Publisher
Nature Publishing Group
Citation
Gene Therapy 2010;17:95-104
Keywords
dental implantplatelet-derived growth factorregenerative medicinevirus delivery
Abstract
Platelet-derived growth factor-BB (PDGF-BB) stimulates repair of healing-impaired chronic wounds such as diabetic ulcers and periodontal lesions. However, limitations in predictability of tissue regeneration occur due, in part, to transient growth factor bioavailability in vivo. Here, we report that gene delivery of PDGF-B stimulates repair of oral implant extraction socket defects. Alveolar ridge defects were created in rats and were treated at the time of titanium implant installation with a collagen matrix containing an adenoviral (Ad) vector encoding PDGF-B (5.5 × 108 or 5.5 × 109 pfu ml 1), Ad encoding luciferase (Ad-Luc; 5.5 × 109 pfu ml 1; control) or recombinant human PDGF-BB protein (rhPDGF-BB, 0.3 mg ml 1). Bone repair and osseointegration were measured through backscattered scanning electron microscopy, histomorphometry, micro-computed tomography and biomechanical assessments. Furthermore, a panel of local and systemic safety assessments was performed. Results indicated that bone repair was accelerated by Ad-PDGF-B and rhPDGF-BB delivery compared with Ad-Luc, with the high dose of Ad-PDGF-B more effective than the low dose. No significant dissemination of the vector construct or alteration of systemic parameters was noted. In summary, gene delivery of Ad-PDGF-B shows regenerative and safety capabilities for bone tissue engineering and osseointegration in alveolar bone defects comparable with rhPDGF-BB protein delivery in vivo. © 2010 Macmillan Publishers Limited All rights reserved.
ISSN
0969-7128
Language
English
URI
https://hdl.handle.net/10371/74086
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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