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Effects of blood contamination on microtensile bond strength to dentin of three self-etch adhesives

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dc.contributor.authorChang, S. W.-
dc.contributor.authorCho, B. H.-
dc.contributor.authorLim, R. Y.-
dc.contributor.authorKyung, S. H.-
dc.contributor.authorPark, D. S.-
dc.contributor.authorOh, T. S.-
dc.contributor.authorYoo, H. M.-
dc.date.accessioned2011-10-13T04:09:45Z-
dc.date.available2011-10-13T04:09:45Z-
dc.date.issued2010-05-
dc.identifier.citationOperative Dentistry 35(3) 330-336en
dc.identifier.issn0361-7734-
dc.identifier.urihttps://hdl.handle.net/10371/74088-
dc.description.abstractThis study evaluated the effects of blood contamination and decontamination methods during différent steps of bonding procedures on the microtensile bond strength of two-step self-etch adhesives to dentin. Sixty extracted human molars were ground flat to expose occlusal dentin. The 60 molars were randomly assigned to three groups, each treated with a different twostep self-etch adhesive: Clearfil SE Bond, AdheSE and Tyrian SPE. In turn, these groups were subdivided into five subgroups (n=20), each treated using different experimental conditions as follows: control group-no contamination; contamination group 1-CG1: primer application/ contamination/primer re-application; contamination group 2-CG2: primer application/contamination/wash/dry/primer re-application; contamination group 3-CG3: primer application/adhesive application/light curing/contamination/ adhesive re-application/light curing; contamination group 4-CG4: primer application/adhesive application/light curing/contamination/wash/ dry/adhesive re-application/light curing. Composite buildup was performed using Z250. After 24 hours of storage in distilled water at 37°C, the bonded specimens were trimmed to an hourglass shape and serially sectioned into slabs with 0.6 mm2 cross-sectional areas. Microtensile bond strengths (MTBS) were assessed for each specimen using a universal testing machine. The data were analyzed by two-way ANOVA followed by a post hoc LSD test. SEM evaluations of the fracture modes were also performed. The contaminated specimens showed lower bond strengths than specimens in the control group (p<0.05), with the exception of CG1 in the Clearfil SE group and CG2 and CG3 in the Tyrian SPE group. Among the three self-etch adhesives, the Tyrian SPE group exhibited a significantly lower average MTBS compared to the Clearfil SE Bond and AdheSE (p<0.05) groups. Based on the results of the current study, it was found that blood contamination reduced the MTBS of all three self-etch adhesives to dentin, and water-rinsing was unable to overcome the effects of blood contamination.en
dc.language.isoenen
dc.publisherOperative Dentistryen
dc.titleEffects of blood contamination on microtensile bond strength to dentin of three self-etch adhesivesen
dc.typeArticleen
dc.identifier.doi10.2341/09-244-L-
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