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Expression profiling of ETS and MMP factors in VEGF-activated endothelial cells: Role of MMP-10 in VEGF-induced angiogenesis

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dc.contributor.authorHeo, Sun-Hee-
dc.contributor.authorChoi, Young-Jin-
dc.contributor.authorRyoo, Hyun-Mo-
dc.contributor.authorCho, Je-Yoel-
dc.date.accessioned2011-10-13T05:46:36Z-
dc.date.available2011-10-13T05:46:36Z-
dc.date.issued2010-09-
dc.identifier.citationJ Cell Physiol 224:734–742en
dc.identifier.issn0021-9541-
dc.identifier.urihttps://hdl.handle.net/10371/74101-
dc.description.abstractIn the process of angiogenesis, working of many transcription factors at the proper time is important to activate angiogenesis-related genes such as cytokine, matrix protease and adhesion molecules. In this study, we searched for Ets transcription factors and matrix metalloproteinases (MMPs) that respond to VEGF in endothelial cells. We first analyzed the expression of 27 human Ets factors and 15 human MMPs in VEGF-treated human umbilical vein endothelial cells (HUVEC) using quantitative RT-PCR. The most abundant Ets factors in HUVEC were ETS-1, Fli-1, ERP/NET/ELK3, and ERG. MMP-1, -2, -10, -11, -14, -15, and -16 were also detected in HUVEC. We also found that ETV-1, Fli-1, ERG, MMP-1, -3, -7, -8, -9, -10, -13, and -19 expression is up-regulated more than 1.5-fold in HUVEC after 2 h of VEGF treatment. In addition, the expression of MMP-10 induced by VEGF remained twofold higher for 24 h compared to non-treated control. The elevation of MMP10 mRNA and protein levels was confirmed to be both time- and dosage-dependent. In addition, MMP-10 transcription was mediated by Ets-1 but not ERP/NET/ELK3. The inhibition of PI3K and MAPK inhibited VEGF-induced MMP-10 expression. Furthermore, transfection of MMP-10 siRNA inhibited VEGF-induced migration and tube formation in HUVEC, and it also inhibited vessel formation in matrigel plugs in vivo. In conclusion, our study demonstrated induction of MMP-10 by VEGF in HUVEC and supports an angiogenic role for MMP-10 in response to VEGF stimulation in vitro and in vivo.en
dc.description.sponsorshipContract grant sponsor: Korea Science and Engineering Foundation (KOSEF);
Contract grant numbers: 2006-2004608, 2008-0062528.
en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.titleExpression profiling of ETS and MMP factors in VEGF-activated endothelial cells: Role of MMP-10 in VEGF-induced angiogenesisen
dc.typeArticleen
dc.contributor.AlternativeAuthor허선희-
dc.contributor.AlternativeAuthor최영진-
dc.contributor.AlternativeAuthor류현모-
dc.contributor.AlternativeAuthor조제열-
dc.identifier.doi10.1002/jcp.22175-
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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