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Toll-like receptor3-mediated induction of chemokines in salivary epithelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, Jingchao | - |
dc.contributor.author | Jeong, Mi Young | - |
dc.contributor.author | Bae, Ji Hyun | - |
dc.contributor.author | Shin, Yong Hwan | - |
dc.contributor.author | Jin, Meihong | - |
dc.contributor.author | Hang, Sung Min | - |
dc.contributor.author | Lee, Jeong Chai | - |
dc.contributor.author | Lee, Sung Joong | - |
dc.contributor.author | Park, Kyungpyo | - |
dc.date.accessioned | 2011-10-14T05:58:53Z | - |
dc.date.available | 2011-10-14T05:58:53Z | - |
dc.date.issued | 2010-08 | - |
dc.identifier.citation | Korean J Physiol Pharmacol 2010;14:235-240 | en |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://hdl.handle.net/10371/74156 | - |
dc.description.abstract | Toll-like receptors (TLRs) functionally expressed in salivary epithelial cells, but their roles remain elusive. Among TLRs family, TLR3 is activated by dsRNA, a byproduct of viral infection. The aim of this study was to investigate the role of TLR3 in the inflammatory immune responses using HSG cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and ELISA were performed to identify expression of TLRs and TLR3-mediated chemokine inductions. The chemotaxis assay of activated T lymphocytes was also performed. Treatment of HSG cells with polyinosinic: polycytidylic acid (poly(LC)) significantly increased interferon- γ -inducible protein 10 (IP-10), interferon-inducible T-cell α chemoattractant (I-TAC), and regulated on activation, normal T-cells expressed and secreted (RANTES) gene expressions in a concentration-dependent manner. Anti-TLR3 antibody blocked the increases of IP-10 and I-TAC genes. Poly(I:C)-induced increases of IP-10 and I-TAC were also confirmed at protein levels from cell lysates, but their release into extracellular medium was detected only in IP-10. We found that the culture media from HSG cells stimulated with poly(I:C) significantly increases T lymphocyte migration. Our results suggest that TLR3 plays an important role in chemokine induction, particularly IP-10, in salivary epithelial cells. | en |
dc.description.sponsorship | This work was supported by the Korea Science & Engineering Foundation, through the Oromaxillofacial Dysfunction Research Center for the Elderly (R13-2008-008-01001-0) at Seoul National University in Korea and Medi-Future company. | en |
dc.language.iso | en | en |
dc.publisher | The Korean Physiological Society & the Korean | en |
dc.subject | Toll-like receptors | en |
dc.subject | HSG cells | en |
dc.subject | Chemokine | en |
dc.subject | Poly(I:C) | en |
dc.subject | IP-10 | en |
dc.title | Toll-like receptor3-mediated induction of chemokines in salivary epithelial cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정미영 | - |
dc.contributor.AlternativeAuthor | 배지현 | - |
dc.contributor.AlternativeAuthor | 신용환 | - |
dc.contributor.AlternativeAuthor | 황성민 | - |
dc.contributor.AlternativeAuthor | 이정채 | - |
dc.contributor.AlternativeAuthor | 이성중 | - |
dc.contributor.AlternativeAuthor | 박경표 | - |
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