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Ankyrin Repeat-Rich Membrane Spanning/Kidins220 Protein Interacts with Mammalian Septin 5

Cited 12 time in Web of Science Cited 12 time in Scopus
Authors
Park, Han Jeong; Park, Hwan-Woo; Lee, Shin-Jae; Carlos Arevalo, Juan; Park, Young-Seok; Lee, Seung-Pyo; Paik, Ki-Suk; Chao, Moses V.; Chang, Mi-Sook
Issue Date
2010-08-01
Publisher
Korean Society for Molecular and Cellular Biology
Citation
Molecules and Cells Vol.30 No.2, pp. 143-148
Keywords
adaptor proteinneurotrophinreceptorseptinsignal transduction
Abstract
Neurotrophin receptors utilize specific adaptor proteins to activate signaling pathways involved in various neuronal functions, such as neurite outgrowth and cytoskeletal remodeling. The Ankyrin-Repeat Rich Membrane Spanning (ARMS)/kinase D-interacting substrate-220 kDa (Kidins220) serves as a unique downstream adaptor protein of Trk receptor tyrosine kinases. To gain insight into the role of ARMS/Kidins220, a yeast two-hybrid screen of a rat dorsal root ganglion library was performed using the C-terminal region of ARMS/Kidins220 as bait. The screen identified a mammalian septin, Septin 5 (Sept5), as an interacting protein. Co-immunoprecipitation using lysates from transiently transfected HEK-293 cells revealed the specific interaction between ARMS/Kidins220 and Sept5. Endogenous ARMS/Kidins220 and Sept5 proteins were colocalized in primary hippocampal neurons and were also predominantly expressed at the plasma membrane and in the tips of growing neurites in nerve growth factor-treated PC12 cells. Mapping of Sept5 domains important for ARMS/Kidins220 binding revealed a highly conserved N-terminal region of Sept5. The direct interaction between ARMS/Kidins220 and Sept5 suggests a possible role of ARMS/Kidins220 as a functional link between neurotrophin receptors and septins to mediate neurotrophin-induced intracellular signaling events, such as neurite outgrowth and cytoskeletal remodeling.
ISSN
1016-8478(print)
0219-1032(online)
Language
English
URI
http://hdl.handle.net/10371/74165
DOI
https://doi.org/10.1007/s10059-010-0099-7
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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