Browse

Rheological properties of hyaluronic acid and its effects on salivary enzymes and candida

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Park, M-S; Chang, J-Y; Kang, J-H; Park, K-P; Kho, H-S
Issue Date
2010-05
Publisher
John Wiley & Sons
Citation
Oral Diseases (2010) 16, 382–387
Keywords
hyaluronic acidsaliva substituteviscositywettabilitylysozymeperoxidase
Abstract
Objective: To investigate the viscosity and wettability of hyaluronic acid (HA), its effects on lysozyme and peroxidase activities, and its candidacidal activity. Materials and methods: Human whole saliva, HA, hen egg-white lysozyme (HEWL), and bovine lactoperoxidase (bLPO) were used. Viscosity was measured with a cone-and-plate digital viscometer, while wettability was determined by measuring the contact angle. Lysozyme activity was determined by the turbidimetric method. Peroxidase activity was determined with NbsSCN assay. Candidacidal activity was determined by comparing colony forming units. Results: The viscosity of HA solutions was proportional to its concentration, with 0.05 mg ml-1 of HA in distilled water or 0.5 mg ml-1 in simulated salivary buffer displaying similar viscosity values to stimulated whole saliva. The contact angle of HA solutions showed no significant differences according to the tested materials and tested HA concentrations. Contact angles of HA solutions on acrylic resin were higher than those of human saliva. HA did not affect lysozyme or peroxidase activities of whole saliva as well as HEWL or bLPO activities. HA also showed no candidacidal activity. Conclusions: The viscoelastic properties of HA compared with human saliva were objectively confirmed, indicating a vital role for HA in the development of effective salivary substitutes.
ISSN
1354-523X
Language
English
URI
https://hdl.handle.net/10371/74170
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse