S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium
- Issue Date
- Springer Verlag
- J. Microbiol. 43,204-208
- dental plaque ; mitis-salivarius bacitracin agar ; non-mutans streptococci organism ; 16S rDNA
- The objective of this study was to both isolate and identify non-mutans streptococci organisms (non-
MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The
dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on
MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as
with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects
had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects,
12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined
to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies).
These results strongly suggest that a new selective medium will be required for the reliable isolation
of mutans streptococci.
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