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Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium

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Issue Date
2005-04
Publisher
Springer Verlag
Citation
J. Microbiol. 43,204-208
Keywords
dental plaquemitis-salivarius bacitracin agarnon-mutans streptococci organism16S rDNA
Abstract
The objective of this study was to both isolate and identify non-mutans streptococci organisms (non-
MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The
dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on
MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as
with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects
had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects,
12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined
to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies).
These results strongly suggest that a new selective medium will be required for the reliable isolation
of mutans streptococci.
ISSN
1976-3794
Language
English
URI
https://hdl.handle.net/10371/74247
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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