S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Curcuma xanthorrhiza oil 및 죽염배합세치제의 치은염억제효과
Gingivitis suppression effect of the de novo dentifrice containing Curcuma xanthorrhiza, bamboo salt and various additives
- 황수정; 김상년; 장석윤; 하원호; 김인수; 진보형; 백대일; 김현덕
- Issue Date
- J Korean Acad Dent Health 2005;29(4):451-462
- 세치제; 실험치은염모형; 치은염; Curcuma xanthorrhiza; Curcuma xanthorrhiza oil; dentifrice; experimental gingivitis model; gingivitis; Matrix metalloproteinase
- Objectives: This study aimed to evaluate the gingivitis suppression effect of the dentifrice containing
bamboo salt, Curcuma Xanthorrhiza, Dipotassium glycyrrhizinate, Ursodeoxycholic acid and various
additives clinically and to estimate Matrix metalloproteinases in Etro and in Evo.
Methods: 1. In vitro : PDL cells which were cultured succeeding a generation were treated by the
mixture of Curcuma Xanthorrhiza, Dipotassium glycyrrhizinate and Ursodeoxycholic acid. After the
treatment of porphyromonas gingivallis culture fluid(l5m/㎖), they were cultured for 3 days. Gelatin
zymography quantified the amount of MMP-2. 2. In vivo clinical intervention study : This study was
designed as a randomized blined clinical trial with factorial design using split mouth method and
experimental gingivitis model. 3. Subjects : 57 Korean male adults participated this study by a written
consent. All subjects were randomized into 3 groups. Twice-a-day tooth brushing was recommended
for 5 weeks, and plaque index and gingival index were evaluated at the baseline, lst, 3rd, 5th weeks.
In Toothshield model, the Toothshield was used for 3 weeks to evaluate gingival index and collect
gingival crevicular fluid in the shielded sulcus. For the recovery the usual tooth-brushing was
recommended for 2 weeks. Repeated measure ANCOVA was used for the time series comparison and
ANCOVA for the comparison between the groups.
Results: The de novo dentifrice suppressed the amount of MMP-2 dose-dependently in vitro. In toothbrushing
model using the dentifrices, the de novo toothpaste reduced gingival index more 49.9%
compared with placebo(p=0.024). In toothshield model, the de novo toothpaste reduced more 38.1%
for gingival index and more 13.4% for MMP-9 than placebo, which were not statistically significant.
Conclusion: The dentifrice containing bamboo salt, Curcuma Xanthorrhiza, Dipotassium
glycyrrhizinate, Ursodeoxycholic acid and various additives could be a useful dentifrice for reducing