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Cloning and sequence analysis of the gene encoding the crystalline surface layer protein of Rickettsia typhi
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- Authors
- Issue Date
- 1993-10
- Publisher
- Elsevier
- Citation
- Gene 133 (1993) 129-133
- Abstract
- The nucleotide sequence of the gene (slp7) encoding the crystalline surface layer protein (SLP) of Rickettsia typhi was
determined. The slpTgene consists of 4935 bp coding for a 1645-amino-acid (aa) protein containing a predicted signal
peptide at the N terminus. The size of the predicted SLP exceeds the observed size (135 kDa) on SDS-PAGE. The
N-terminal aa sequence of the 32-kDa protein of R. typhi reported by Hackstadt et al. [Infect. Immun. 60 (1992)
159-1651 was found in the C-terminal portion of the deduced aa sequence, suggesting that the product of slpT is
processed into the mature SLP and the 32-kDa protein.
- ISSN
- 0378-1119
- Language
- English
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