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Alteration of DNA Synthesis and p53 Level by Modulators of Adenylate Cyclase System and Other Second Messenger Systems in Human Oral Keratinocytes

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dc.contributor.authorKim, Myong Soo-
dc.contributor.authorMin, Byung-Moo-
dc.contributor.authorKim, Gwan-Shik-
dc.date.accessioned2011-10-18T08:02:55Z-
dc.date.available2011-10-18T08:02:55Z-
dc.date.issued1994-
dc.identifier.citationJ. Oral Bio. 18:13-22,1994en
dc.identifier.issn1225-6390-
dc.identifier.urihttps://hdl.handle.net/10371/74292-
dc.description.abstractTo study the role of adenylate cyclase system and other mediators of signal transduction pathway in the cell cycle arrest which provides enough time for the cells to repair damaged DNA, we examined the cellular response of primarily cultured human oral keratinocytes (HOKs) to UV irradiation, UV irradiation reduced replicative DNA synthesis of HOKs in a dose-drpendent manner without significant changes in viability. Dibutyryl cAMP(5 uM) and forskotin (5 uM) partly inhibited the reduction in replicative DNA failed to inhibit the reduction in replicative DNA synthesis at 12th hour after irradiation. At 24th hour after irradiation, none of the agents inhibited the suppression of DNA synthesis. The reduction in DNA synthesis was accompanied by marked p53 induction, which was completely blocked by dibutyryl cAMP (5 uM) forskolin (5 uM), IBMX (5 uM) and dibutyryl cGMP (5 uM). Treatment of TPA (5 uM), staurosporin (10 uM) and dexamethasone (1 uM) reduced DNA synthesis in HOKs ana prompted death of the cells. Those drugs, however. did not induce p53 indicating that different mechanism from that for UV irradiation may be involved in the reduction of replicative DNA synthesis. Increased reparative DNA synthesis induced by UV irradiation was suppressed by forskolin. Our results suggest that cAMP may be involved in DNA damage-induced cell cycle arrest and DNA repair, and cAMP and cGMP may be associated with blockade of p53 induction.en
dc.description.sponsorshipThis work was supported by a grant no.01-94-267 from the Seoul National University Hospital Research Fund.en
dc.language.isoenen
dc.publisherThe Korean Academy of Oral Biologyen
dc.subjectcAMPen
dc.subjectUV radiationen
dc.subjectkeratinocytesen
dc.subjectp53en
dc.titleAlteration of DNA Synthesis and p53 Level by Modulators of Adenylate Cyclase System and Other Second Messenger Systems in Human Oral Keratinocytesen
dc.typeArticleen
dc.contributor.AlternativeAuthor김명수-
dc.contributor.AlternativeAuthor민병무-
dc.contributor.AlternativeAuthor김관식-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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