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Swelling-activated Cl- Channels in Human Salivary Gland Acinar Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, Gehoon | - |
dc.contributor.author | Sim, Jaehyun | - |
dc.contributor.author | Kim, Soung Min | - |
dc.contributor.author | Lee, Jong-Ho | - |
dc.contributor.author | Chun, Gae-Sig | - |
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Park, Kyungpyo | - |
dc.date.accessioned | 2011-10-20T01:20:49Z | - |
dc.date.available | 2011-10-20T01:20:49Z | - |
dc.date.issued | 2009-09 | - |
dc.identifier.citation | Int. J. Oral Biol. 2009;34(3):151-155 | en |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://hdl.handle.net/10371/74363 | - |
dc.description.abstract | The role of Cl
- channels in regulatory volume decrease (RVD) in human salivary gland acinar cells was examined using a whole-cell patch clamp technique. Human tissues were obtained from healthy volunteers or from patients with oromaxillofacial tumors. During the measurements, K+-free solutions were employed to eliminate contamination of whole-cell conductance by K+ currents. When the cells were exposed to a 70% hypotonic solution, outward-rectifying currents, which were not observed in the resting state, were found to have significantly increased both in human labial and parotid gland acinar cells. The amplitudes of the currents were reduced in a low Cl - bath solution. Furthermore, the addition of 100 μM 5-Nitro-2- (3-phenyl propylamino) benzoic acid (NPPB) or 100 μM 4,4'-diisothio cyanatostilbene -2,2'-disulphonic acid (DIDS), known to partially block Cl - channels, significantly inhibited these currents. Its outward-rectifying current profile, shift in reversal potential in a low Cl - bath solution and pharmacological properties suggest that this is a Ca 2+-independent, volume activated Cl - current. We conclude therefore that volume activated Cl - channels play a putative role in RVD in human salivary gland acinar cells. | en |
dc.description.sponsorship | This work was supported by the Korea Science & Engineering Foundation (KOSEF) grant funded by Korea government(R13-2008-008-01001-0) through the Oromaxillofacial Dysfunction Research Center for the Elderly at Seoul National University. | en |
dc.language.iso | ko | en |
dc.publisher | Korean Acadamy of Oral Biology | en |
dc.subject | Cl- channels | en |
dc.subject | Regulatory volume decrease(RVD) | en |
dc.subject | Hypotonic | en |
dc.subject | NPPB | en |
dc.subject | DIDS | en |
dc.title | Swelling-activated Cl- Channels in Human Salivary Gland Acinar Cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정재훈 | - |
dc.contributor.AlternativeAuthor | 심재현 | - |
dc.contributor.AlternativeAuthor | 김성민 | - |
dc.contributor.AlternativeAuthor | 이종호 | - |
dc.contributor.AlternativeAuthor | 천재식 | - |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 박경표 | - |
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