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Activation of Vanilloid Receptor 1 (VR1) by Eugenol

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Authors

Yang, B.H; Piao, Z.G.; Kim, Y.-B.; Lee, C.-H.; Lee, J.K.; Park, K.; Kim, J.S.; Oh, S.B.

Issue Date
2003-10
Publisher
SAGE Publications
Citation
J Dent Res 82:781-785
Keywords
eugenolcapsaicintrigeminal ganglionHEK 293 cellsvanilloid receptor 1
Abstract
The structural similarity of eugenol with capsaicin suggests that these two agents may share molecular mechanisms to produce their effects. We investigated the effects of eugenol in comparison with those of capsaicin using whole-cell patch clamp and Fura-2-based calcium-imaging techniques in a heterologous expression system and with sensory neurons. In vanilloid receptor 1 (VR1)-expressing human embryonic kidney (HEK) 293 cells and trigeminal ganglion (TG) neurons, eugenol activated inward currents, whereas capsazepine, a competitive VR antagonist, and ruthenium red (RR), a functional VR antagonist, completely blocked eugenol-induced inward currents. Moreover, eugenol caused elevation of [Ca2+]i, and this was completely abolished by both capsazepine and ruthenium red in VR1-expressing HEK 293 cells and TG neurons. Our results provide strong evidence that eugenol produces its effects, at least in part, via VR1 expressed by the sensory nerve endings in the teeth.
ISSN
1544-0591
Language
English
URI
https://hdl.handle.net/10371/74364
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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