Antiobesity effect of trans-10, cis-12-conjugated linoleic acid-producing Lactobacillus plantarum PL62 on diet-induced obese mice

Abstract

Aims: To observe the antiobesity activity of trans-10, cis-12-conjugated linoleic acid (CLA)-producing lactobacillus in mice. Methods and Results: Lactobacillus plantarum PL62, which can grow in the presence of linoleic acid, was selected and studied. The culture supernatant of Lact. plantarum PL62 contained trans-10, cis-12-conjugated linoleic acid (6·4 μg ml⁻¹), and the crude enzyme prepared from washed cells produced trans-10, cis-12 CLA (1395 μg mg⁻¹ protein). Lact. plantarum PL62 reduced the weights of epididymal, inguinal, mesenteric, and perirenal white adipose tissues and significantly reduced the blood levels of total glucose and body weights of mice ( P < 0·01). Conclusions: trans-10, cis-12-CLA-producing Lact. plantarum PL62 can exert the same antiobesity activity as trans-10, cis-12-CLA in mice. Significance and impact of the study: trans-10, cis-12-CLA-producing Lactobacillus can be a replacement for CLA for obesity treatment via the continuous production of trans-10, cis-12-CLA. The results provide a novel opportunity to develop foods with antiobesity activity.