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Evaluation of phoP and rpoS mutants of Salmonella enterica serovar Typhi as attenuated typhoid vaccine candidates: virulence and protective immune responses in intranasally immunized mice

Cited 13 time in Web of Science Cited 12 time in Scopus
Authors
Lee, Hui-Young; Cho, Sun-A; Lee, In-Soo; Park, Jong-Hwan; Seok, Seung-Hyeok; Baek, Min-Won; Kim, Dong-Jae; Lee, Seok-Ho; Hur, Sook-Jin; Ban, Sang-Ja; Lee, Yoo-Kyoung; Han, Yang-Keum; Cho, Young-Keun; Park, Jae Hak
Issue Date
2007-08-24
Publisher
Wiley-Blackwell
Citation
FEMS Immunol Med Microbiol 51: 310-318
Keywords
Salmonella enterica serovar strain TyphiinvasivenessattenuationrpoSphoPmouse
Abstract
The attenuation and immunoenhancing effects of rpoS and phoPSalmonella enterica serovar strain Typhi (Salmonella typhi) mutants have not been compared. Here, three S. typhi deletion mutants (phoP, rpoS, and rpoS–phoP double mutant) are constructed and these mutants are characterized with respect to invasiveness, virulence, and protective immune response compared with wild-type Ty2. It was found that phoP and phoP–rpoS deletion mutants are less invasive to HT-29 cells than the wild-type Ty2 and the rpoS single-deleted strain. The LD50 of immunized mice was higher for phoP than for rpoS mutants, and the highest for the phoP–rpoS double mutant. In addition, all S. typhi mutants showed an increase in the specific serum IgG levels and T-cell-mediated immunity, and showed equal protection abilities against a wild-type Ty2 challenge after two rounds of immunization in BALB/c mice. It is concluded that phoP genes appear to play a more important role than rpoS genes in both cellular invasion and virulence of S. typhi, but not in immunogenicity in mice. Furthermore, the data indicate that the phoP–rpoS double mutant may show promise as a candidate for an attenuated typhoid vaccine.
ISSN
0928-8244
Language
English
URI
http://hdl.handle.net/10371/7464
DOI
https://doi.org/10.1111/j.1574-695X.2007.00307.x
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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