Publications
Detailed Information
Hyperoxidized Peroxiredoxins and Glyceraldehyde-3-Phosphate Dehydrogenase Immunoreactivity and Protein Levels are Changed in the Gerbil Hippocampal CA1 Region After Transient Forebrain Ischemia
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, In Koo | - |
dc.contributor.author | Yoo, Ki-Yeon | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Choi, Jung Hoon | - |
dc.contributor.author | Lee, In Se | - |
dc.contributor.author | Won, Moo Ho | - |
dc.date.accessioned | 2009-08-24T08:35:17Z | - |
dc.date.available | 2009-08-24T08:35:17Z | - |
dc.date.issued | 2007-04-25 | - |
dc.identifier.citation | Neurochem Res 32:1530-1538 | en |
dc.identifier.issn | 0364-3190 (print) ) | - |
dc.identifier.issn | 1573-6903 (online | - |
dc.identifier.uri | https://hdl.handle.net/10371/7607 | - |
dc.description.abstract | Oxidative stress is a major pathogenic event occurring in several brain disorders and is a major cause of brain damage due to ischemia/reperfusion. Thiol proteins are easily oxidized in cells exposed to reactive oxygen species (ROS). In the present study, we investigated transient ischemia-induced chronological changes in hyperoxidized peroxiredoxins (Prx-SO3) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH-SO3) immunoreactivity and protein levels in the gerbil hippocampus induced by 5 min of transient forebrain ischemia. Weak Prx-SO3 immunoreactivity is detected in the hippocampal CA1 region of the sham-operated group. Prx-SO3 immunoreactivity was significantly increased 12 h and 1 day after ischemia/reperfusion, and the immunoreactivity was decreased to the level of the sham-operated group 2 days after ischemia/reperfusion. Prx-SO3 immunoreactivity in the 4 days post-ischemia group was increased again, and the immunoreactivity was expressed in glial components for 5 days after ischemia/reperfusion. GAPDH-SO3 immunoreactivity was highest in the CA1 region 1 day after ischemia/reperfusion, the immunoreactivity was decreased 2 days after ischemia/reperfusion. Four days after ischemia/reperfusion, GAPDH-SO3 immunoreactivity increased again, and the immunoreactivity began to be expressed in glial components from 5 days after ischemia/reperfusion. Prx-SO3 and GAPDH-SO3 protein levels in the ischemic CA1 region were also very high 12 h and 1 day after ischemia/reperfusion and returned to the level of the sham-operated group 3 days after ischemia/reperfusion. Their protein levels were increased again 5 days after ischemia/reperfusion. In conclusion, Prx-SO3 and GAPDH-SO3 immunoreactivity and protein levels in the gerbil hippocampal CA1 region are significantly increased 12 h-24 h after ischemia/reperfusion and their immunoreactivity begins to be expressed in glial components from 4 or 5 days after ischemia/reperfusion. | en |
dc.description.sponsorship | The authors would like to thank Mr. Suek Han,
Seung Uk Lee and Ms. Hyun Sook Kim for their technical help in this study. This study was supported by a grant of the Korean Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A050742). | en |
dc.language.iso | en | - |
dc.publisher | Springer Verlag | en |
dc.subject | Peroxiredoxins | en |
dc.subject | Glyceraldehyde-3-phosphate dehydrogenase | en |
dc.subject | Hyperoxidation | en |
dc.subject | Reactive oxygen species | en |
dc.subject | Cerebral ischemia | en |
dc.title | Hyperoxidized Peroxiredoxins and Glyceraldehyde-3-Phosphate Dehydrogenase Immunoreactivity and Protein Levels are Changed in the Gerbil Hippocampal CA1 Region After Transient Forebrain Ischemia | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 황인구 | - |
dc.contributor.AlternativeAuthor | 유기연 | - |
dc.contributor.AlternativeAuthor | 김대원 | - |
dc.contributor.AlternativeAuthor | 최정훈 | - |
dc.contributor.AlternativeAuthor | 이인세 | - |
dc.contributor.AlternativeAuthor | 원무호 | - |
dc.identifier.doi | 10.1007/s11064-007-9345-6 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.