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High expression of large-conductance Ca(2+)-activated K(+) channel in the CD133(+) subpopulation of SH-SY5Y neuroblastoma cells

Cited 18 time in Web of Science Cited 20 time in Scopus
Authors
Park, Ji Hyun; Park, Su Jung; Jung, Kyoung Hwa; Chung, Mi Kyung; Choi, Mi Ran; Chai, Young Gyu; Park, Kyoung Sun; Kim, Sung Joon; Kim, Yangmi
Issue Date
2010-06-04
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS; Vol.396 3; 637-642
Keywords
NeuroblastomaReal-time PCRIntracellular Ca(2+)Cancer stem cellsCa(2+)-activated K(+) channelIon channels
Abstract
Solid tumors contain a population of cancer stem cells (CSCs), and CD133 is widely used as a CSCs marker. We investigated the differences between CD133(+) and CD133(-) cells from the neuroblastoma cell line SH-SY5Y in terms of the expressions of voltage-gated ion channels. A CD133 enriched (>60%) population was isolated, and a subsequent whole-cell voltage-clamp study showed that these cells predominantly express TEA-sensitive outward K(+) currents (I(K,TEA)) and TTX-sensitive voltage-gated inward Na(+) currents (I(Na)). Cell-attached single channel recordings demonstrated higher density of large-conductance (155 pS) channel in CD133(+) cells than in CD133(-) cells. The TEA-sensitivity and single channel conductance indicated the large-conductance Ca(2+)-activated K(+) channels (BK(Ca)). Furthermore, RT-PCR analysis of 22 transcripts of voltage-gated ion channels in SH-SY5Y cells showed the expressions of Cav1.3, Kir2.1, Kv1.4, Kv2.1, Kv4.2, Kv7.1, BK(Ca), and Nav1.7, and those of BK(Ca) and Nav1.7 were higher in CD133+ than in CD133- cells. In addition, the increase of cytosolic Ca(2+) concentration ([Ca(2+)](c)) in response to ionomycin (a Ca(2+) ionophore) was higher and more sustained in CD133(+) than in CD133(-) cells. Plausibly membrane hyperpolarization via BK(Ca) might be responsible for the augmented Ca(2+) influx observed in CD133(+). cells. The physiological implications of the differential expression of BK(Ca) and Nav1.7 in CSCs require further investigation. (C) 2010 Elsevier Inc. All rights reserved.
ISSN
0006-291X
Language
English
URI
http://hdl.handle.net/10371/76221
DOI
https://doi.org/10.1016/j.bbrc.2010.04.142
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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