S-Space College of Medicine/School of Medicine (의과대학/대학원) Laboratory Medicine (검사의학전공) Journal Papers (저널논문_검사의학전공)
Accuracy of Platelet Counting by Automated Hematologic Analyzers in Acute Leukemia and Disseminated lntravascular Coagulation Potential Effects of Platelet Activation
- Kim, Seon Young; Kim, Ji-Eun; Han, Kyou-Sup; Kim, Hyun Kyung; Toh, Cheng Hock
- Issue Date
- AMER SOC CLINICAL PATHOLOGY
- AMERICAN JOURNAL OF CLINICAL PATHOLOGY; Vol.134 4; 634-647
- Platelet count; Acute leukemia; Disseminated intravascular coagulation; Platelet activation
- Platelet counting in patients with acute leukemia or disseminated intravascular coagulation (DIC) may have a risk for erroneous counts owing to the presence of nonplatelet particles or platelet activation. We evaluated automated platelet counting methods using the Abbott Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA), Sysmex XE-2100 (Sysmex, Kobe, Japan), AD VIA 2120 (Siemens Diagnostics, Tarrytown, NY), and Beckman Coulter LH 750 (Beckman Coulter, Miami, FL) compared with the international reference method (IRM). Automated platelet counting methods were inaccurate compared with the IRM, without evidence of interfering nonplatelet particles. It is interesting that platelet activation markers were associated with DIC severity and erroneous platelet counting, suggesting that platelet activation is a potential source of inaccuracy. Furthermore, the artifactual in vitro platelet activation induced a high degree of intermethod variation in platelet counts. The inaccuracy of automated platelet counts increased the risk for misdiagnosis of DIC. More attention needs to be given to the accuracy of platelet counts, especially in clinical conditions with florid platelet activation.
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