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Chloride intracellular channel 1 regulates osteoblast differentiation

Cited 20 time in Web of Science Cited 24 time in Scopus
Authors
Yang, Jae-Yeon; Jung, Ju Yeon; Cho, Sun Wook; Choi, Hyung Jin; Kim, Seong Yeon; Jang, Chang Han; Han, Jin; Shin, Chan Soo; Lee, Min Goo; Kim, Hee Joong; Kim, Sang Wan
Issue Date
2009-12
Publisher
ELSEVIER SCIENCE INC
Citation
BONE; Vol.45 6; 1175-1185
Keywords
OsteoblastWntDifferentiationMitochondriaChloride intracellular channel
Abstract
We have identified chloride intracellular channel 1 (CLIC1) through proteomic approach, which was increased in response to canonical wnt signaling while being almost shut-off by adipogenic treatment in mouse mesenchymal C3H10T1/2 cells. We found that CLIC1 was expressed in mouse (MC3T3-E1), rat (ROS 17/2.8 and UMR-106) or human (MG63 and SaOS2) osteoblastic cell lines as well as primary culture of mouse calvarial cells by RT-PCR or Western blot analysis. The expression level of CLIC1 is increased upon treatment of osteogenic medium, whereas it almost disappeared in adipogenic condition, confirming the proteomic data. The expression of CLIC1 was localized mainly in nuclear membrane and vesiculocytoplasmic, the latter of which was colocalized with mitochondria. Retroviral overexpression of CLIC1 did not increase whole-cell current but induces hyperpolarization of mitochondrial membrane potential estimated using the fluorescent dye TMRE. Moreover, overexpression of CLIC1 resulted in increase in osteoblastic differentiation of C3H10T1/2 cells as measured by ALP activities or osteoblastic gene expression (osterix, ALP and osteocalcin), although it did not result in induction of Runx2 transcription activities at mouse osteocalcin (OG2) promoter. Finally, in vitro knock-down of CLIC1 using stable siRNA CLIC1 significantly suppressed osteoblastic differentiation. Taken together, these results suggest that CLIC1 may play a role in the regulation of osteoblastic differentiation from mesenchymal progenitors, although its physiologic role in osteoblasts remains to be determined. (C) 2009 Elsevier Inc. All rights reserved.
ISSN
8756-3282
Language
English
URI
https://hdl.handle.net/10371/76319
DOI
https://doi.org/10.1016/j.bone.2009.08.012
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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