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Locally Delivered Growth Factor Enhances the Angiogenic Efficacy of Adipose-Derived Stromal Cells Transplanted to Ischemic Limbs

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dc.contributor.authorBhang, Suk Ho-
dc.contributor.authorCho, Seung-Woo-
dc.contributor.authorLim, Jae Min-
dc.contributor.authorKang, Jin Muk-
dc.contributor.authorYang, Hee Seok-
dc.contributor.authorPark, Moon Hyang-
dc.contributor.authorYoo, Kyung-Jong-
dc.contributor.authorLanger, Robert-
dc.contributor.authorKim, Byung-Soo-
dc.contributor.authorAnderson, Daniel G.-
dc.contributor.authorJang, Yangsoo-
dc.contributor.authorKim, Hyo-Soo-
dc.contributor.authorSong, Young Soo-
dc.contributor.authorLee, Tae-Jin-
dc.date.accessioned2012-05-24T04:57:07Z-
dc.date.available2012-05-24T04:57:07Z-
dc.date.issued2009-08-
dc.identifier.citationSTEM CELLS; Vol.27 8; 1976-1986ko_KR
dc.identifier.issn1066-5099-
dc.identifier.urihttps://hdl.handle.net/10371/76400-
dc.description.abstractIschemia is a potentially fatal medical event that is associated with as many as 30% of all deaths. Stem cell therapy offers significant therapeutic promise, but poor survival following transplantation to ischemic tissue limits its efficacy. Here we demonstrate that nanosphere-mediated growth factor delivery can enhance the survival of transplanted human adipose-derived stromal cells (hADSCs) and secretion of human angiogenic growth factors per cell, and substantially improve therapeutic efficacy of hADSCs. In vitro, in hypoxic (1% oxygen) and serum-deprived conditions that simulate in vivo ischemia, fibroblast growth factor-2 (FGF2) significantly reduced hADSC apoptosis and enhanced angiogenic growth factor secretion. In vivo, hADSCs delivered intramuscularly into ischemic hind limbs in combination with FGF2 resulted in significant improvements in limb survival and blood perfusion, as well as survival of the transplanted hADSCs and secretion of human angiogenic growth factors (i.e., vascular endothelial growth factor, hepatocyte growth factor, and FGF2). Interestingly, the majority of transplanted hADSCs were localized adjacent to the micro-vessels rather than being incorporated into them, suggesting that their major contribution to angiogenesis might be to increase paracrine secretion of angiogenic growth factors. This study demonstrates the potential of hADSCs in combination with growth factors for use in the treatment of ischemia. STEM CELLS 2009;27:1976-1986ko_KR
dc.language.isoenko_KR
dc.publisherWILEY-BLACKWELLko_KR
dc.subjectHuman adipose-derived stromal cellko_KR
dc.subjectParacrine effectko_KR
dc.subjectTherapeutic angiogenesisko_KR
dc.subjectHind limb ischemiako_KR
dc.subjectLocal deliveryko_KR
dc.subjectFibroblast growth factor-2ko_KR
dc.titleLocally Delivered Growth Factor Enhances the Angiogenic Efficacy of Adipose-Derived Stromal Cells Transplanted to Ischemic Limbsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor방석호-
dc.contributor.AlternativeAuthor조승우-
dc.contributor.AlternativeAuthor임재민-
dc.contributor.AlternativeAuthor강진묵-
dc.contributor.AlternativeAuthor이태진-
dc.contributor.AlternativeAuthor양희석-
dc.contributor.AlternativeAuthor송영수-
dc.contributor.AlternativeAuthor박문향-
dc.contributor.AlternativeAuthor김효수-
dc.contributor.AlternativeAuthor유경종-
dc.contributor.AlternativeAuthor장양수-
dc.contributor.AlternativeAuthor김병수-
dc.identifier.doi10.1002/stem.115-
dc.citation.journaltitleSTEM CELLS-
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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