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Overexpression of α-catenin increases osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cells

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dc.contributor.authorKim, Dohee-
dc.contributor.authorYang, Jae-Yeon-
dc.contributor.authorShin, Chan Soo-
dc.date.accessioned2012-05-24T06:23:58Z-
dc.date.available2012-05-24T06:23:58Z-
dc.date.issued2009-05-15-
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS; Vol.382 4; 745-750ko_KR
dc.identifier.issn0006-291X-
dc.identifier.urihttps://hdl.handle.net/10371/76422-
dc.description.abstractα-and β-Catenin link cadherins to the actin-based cytoskeleton at adherens junctions and regulate cell-cell adhesion. Although roles of cadherins and canonical Wnt-/β-catenin-signaling in osteoblastic differentiation have been extensively studied, the role of α-catenin is not known. Murine embryonic mesenchymal stem cells, C3H10T1/2 cells, were transduced with retrovirus encoding α-catenin (MSCV-alpha-catenin-HA-GFP). In the presence of Wnt-3A conditioned medium or osteogenic medium (β-glycerol phosphate and ascorbic acid), cells overexpressing α-catenin showed enhanced osteoblastic differentiation as measured by alkaline phosphatase (ALP) staining and ALP activity assay compared to cells transduced with empty virus (MSCV-GFP). In addition, mRNA expression of osteocalcin and Runx2 was significantly increased compared to control. Cell aggregation assay revealed that α-catenin overexpression has significantly increased cell-cell aggregation. However, cellular β-catenin levels (total, cytoplasmic-nuclear ratio) and β-catenin-TCF/LEF transcriptional activity did not change by overexpression of α-catenin. Knock-down of α-catenin using siRNA decreased osteoblastic differentiation as measured by ALP assay. These results suggest that α-catenin overexpression increases osteoblastic differentiation by increasing cell-cell adhesion rather than Wnt-/β-catenin-signaling. (C) 2009 Elsevier Inc. All rights reserved.ko_KR
dc.language.isoenko_KR
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEko_KR
dc.subjectα-Cateninko_KR
dc.subjectOsteoblastko_KR
dc.subjectAdhesionko_KR
dc.subjectWntko_KR
dc.subjectCadherinko_KR
dc.titleOverexpression of α-catenin increases osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cellsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor김도희-
dc.contributor.AlternativeAuthor양재연-
dc.contributor.AlternativeAuthor신찬수-
dc.identifier.doi10.1016/j.bbrc.2009.03.100-
dc.citation.journaltitleBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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