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Overexpression of α-catenin increases osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Dohee | - |
dc.contributor.author | Yang, Jae-Yeon | - |
dc.contributor.author | Shin, Chan Soo | - |
dc.date.accessioned | 2012-05-24T06:23:58Z | - |
dc.date.available | 2012-05-24T06:23:58Z | - |
dc.date.issued | 2009-05-15 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS; Vol.382 4; 745-750 | ko_KR |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://hdl.handle.net/10371/76422 | - |
dc.description.abstract | α-and β-Catenin link cadherins to the actin-based cytoskeleton at adherens junctions and regulate cell-cell adhesion. Although roles of cadherins and canonical Wnt-/β-catenin-signaling in osteoblastic differentiation have been extensively studied, the role of α-catenin is not known. Murine embryonic mesenchymal stem cells, C3H10T1/2 cells, were transduced with retrovirus encoding α-catenin (MSCV-alpha-catenin-HA-GFP). In the presence of Wnt-3A conditioned medium or osteogenic medium (β-glycerol phosphate and ascorbic acid), cells overexpressing α-catenin showed enhanced osteoblastic differentiation as measured by alkaline phosphatase (ALP) staining and ALP activity assay compared to cells transduced with empty virus (MSCV-GFP). In addition, mRNA expression of osteocalcin and Runx2 was significantly increased compared to control. Cell aggregation assay revealed that α-catenin overexpression has significantly increased cell-cell aggregation. However, cellular β-catenin levels (total, cytoplasmic-nuclear ratio) and β-catenin-TCF/LEF transcriptional activity did not change by overexpression of α-catenin. Knock-down of α-catenin using siRNA decreased osteoblastic differentiation as measured by ALP assay. These results suggest that α-catenin overexpression increases osteoblastic differentiation by increasing cell-cell adhesion rather than Wnt-/β-catenin-signaling. (C) 2009 Elsevier Inc. All rights reserved. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | ko_KR |
dc.subject | α-Catenin | ko_KR |
dc.subject | Osteoblast | ko_KR |
dc.subject | Adhesion | ko_KR |
dc.subject | Wnt | ko_KR |
dc.subject | Cadherin | ko_KR |
dc.title | Overexpression of α-catenin increases osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김도희 | - |
dc.contributor.AlternativeAuthor | 양재연 | - |
dc.contributor.AlternativeAuthor | 신찬수 | - |
dc.identifier.doi | 10.1016/j.bbrc.2009.03.100 | - |
dc.citation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
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