Browse

Helicobacter pylori genotyping findings from multiple cultured isolates and mucosal biopsy specimens: strain diversities of Helicobacter pylori isolates in individual hosts

Cited 18 time in Web of Science Cited 24 time in Scopus
Authors
Kim, Young Sun; Kim, Nayoung; Kim, Jung Mogg; Kim, Mi Soon; Lee, Mi Kyoung; Kim, Joo Sung; Song, In Sung; Jung, Hyun Chae; Lee, Dong Ho; Park, Ji Hyun
Issue Date
2009-05
Publisher
LIPPINCOTT WILLIAMS & WILKINS
Citation
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY; Vol.21 5; 522-528
Keywords
diversitiesHelicobacter pylorigenotype
Abstract
Objectives To determine whether the genotypes of virulent genes in Helicobacter pylori isolates and mucosal biopsy specimens differ in individuals, and to investigate whether different isolates from single hosts show strain differences. Methods Sixty-one Korean patients with H. pylori infection were enrolled. PCR and DNA sequencing for cagA, vacA, iceA, and oipA were performed using DNA extracted from H. pylori isolates cultured (2.6 H. pylori isolates per host) directly from antral mucosal biopsy specimens. Strain diversities were analyzed in 234 H. pylori isolates obtained from 43 hosts with at least two H. pylori isolates from antrum and body, respectively, and random amplified polymorphic DNA fingerprinting was carried out on isolates obtained from patients who showed genotype diversity. Results The patients with inconsistent genotyping results between H. pylori isolates and mucosal biopsies were as follows: 16.4% for cagA, 19.7% for vacA m, 47.5% for vacA s1, 6.6% for vacA i-region, 34.4% for iceA, and 21.3% for oipA. Genotyping of H. pylori isolates from same hosts showed diversity in 58.1% (25/43 patients). When random amplified polymorphic DNA-PCR fingerprinting was carried out on 104 H. pylori isolates from 19 patients who showed genotype diversity among their isolates, 68.4% (13 of 19 patients) of patients were found to be colonized by multiple H. pylori strains. Conclusion This study shows that the genotypes of virulent genes from biopsy samples produced different results when compared with those obtained from H. pylori isolates, especially for vacA s1, and iceA. In addition, about 60% of our patients were infected by multiple H. pylori strains. Eur J Gastroenterol Hepatol 21:522-528 (c) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.
ISSN
0954-691X
Language
English
URI
https://hdl.handle.net/10371/76440
DOI
https://doi.org/10.1097/MEG.0b013e3283196af0
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse