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Protein overexpression and gene amplification of epidermal growth factor receptor in nonsmall cell lung carcinomas: Comparison of four commercially available antibodies by immunohistochemistry and fluorescence in situ hybridization study
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun Ju | - |
dc.contributor.author | Xu, Xianhua | - |
dc.contributor.author | Choe, Gheeyoung | - |
dc.contributor.author | Chung, Doo Hyun | - |
dc.contributor.author | Lee, Jae Ho | - |
dc.contributor.author | Jheon, Sanghoon | - |
dc.contributor.author | Chung, Jin-Haeng | - |
dc.contributor.author | Sung, Sook-Whan | - |
dc.contributor.author | Lee, Choon-Taek | - |
dc.contributor.author | Seo, Jeong-Wook | - |
dc.date.accessioned | 2012-05-25T08:52:08Z | - |
dc.date.available | 2012-05-25T08:52:08Z | - |
dc.date.issued | 2010-06 | - |
dc.identifier.citation | LUNG CANCER; Vol.68 3; 375-382 | ko_KR |
dc.identifier.issn | 0169-5002 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76497 | - |
dc.description.abstract | Epidermal growth factor receptor (EGFR) overexpression in nonsmall cell lung carcinomas (NSCLC) is variable ranging from 19% to 89% and its prognostic value remains controversial. We tried to investigate (1) EGFR protein expression using four different antibodies, (2) the correlation between protein overexpression and EGFR gene amplification, and (3) the correlation between EGFR genetic status and clinicopathologic features in NSCLC. We examined EGFR protein expression using four different antibodies including Zymed EGFR kit (Clone 31G7), Dako EGFR pharmDx kit (Clone 2-18C9), Dako (Clone H11) and Novocastra (Clone EGFR 113) by immunohistochemical analysis. The protein overexpression was compared to gene amplification status by fluorescence in situ hybridization (FISH). EGFR protein overexpression was observed in 56% of tumors with Zymed EGFR kit, in 51% with Dako EGFR pharmDx kit, in 5% with Dako and in 18% with Novocastra (p = 0.010). Both Zymed and Dako pharmDx kit were more sensitive than the Dako test (clone H11) and Novocastra clone EGFR 113. EGFR overexpression was more prominent in squamous cell carcinomas (SCC) than adenocarcinomas (ADC) (71% vs. 48%, p = 0.001 with Zymed, 61% vs. 45%, p = 0.011 with Dako pharmDx kit; respectively). EGFR FISH-positivity as represented by high polysomy and gene amplification was observed in 45% of the NSCLC patients. Protein expression levels significantly correlated with the gene copy number per tumor cell (p < 0.001). Our data showed a higher percentage of positive cells detected by Zymed and Dako pharmDx tests. The EGFR protein overexpression rate varied from 4% to 72% according to different antibody clones and histologic types. EGFR protein expression detected by Zymecl and Dako pharmDx was significantly associated with a high EGFR gene copy number. (C) 2009 Elsevier Ireland Ltd. All rights reserved. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ELSEVIER IRELAND LTD | ko_KR |
dc.subject | EGFR | ko_KR |
dc.subject | Lung neoplasm | ko_KR |
dc.subject | FISH | ko_KR |
dc.subject | Immunohistochemistry | ko_KR |
dc.title | Protein overexpression and gene amplification of epidermal growth factor receptor in nonsmall cell lung carcinomas: Comparison of four commercially available antibodies by immunohistochemistry and fluorescence in situ hybridization study | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이현주 | - |
dc.contributor.AlternativeAuthor | 최기영 | - |
dc.contributor.AlternativeAuthor | 정두현 | - |
dc.contributor.AlternativeAuthor | 서정욱 | - |
dc.contributor.AlternativeAuthor | 이재호 | - |
dc.contributor.AlternativeAuthor | 이춘택 | - |
dc.contributor.AlternativeAuthor | 전상훈 | - |
dc.contributor.AlternativeAuthor | 성숙환 | - |
dc.contributor.AlternativeAuthor | 정진행 | - |
dc.identifier.doi | 10.1016/j.lungcan.2009.07.014 | - |
dc.citation.journaltitle | LUNG CANCER | - |
dc.description.citedreference | Reinmuth N, 2008, LUNG CANCER, V62, P193, DOI 10.1016/j.lungcan.2008.03.011 | - |
dc.description.citedreference | Felip E, 2008, CLIN CANCER RES, V14, P3867, DOI 10.1158/1078-0432.CCR-07-5186 | - |
dc.description.citedreference | Ahn MJ, 2008, CLIN CANCER RES, V14, P3860, DOI 10.1158/1078-0432.CCR-07-4608 | - |
dc.description.citedreference | Li AR, 2008, J MOL DIAGN, V10, P242, DOI 10.2353/jmoldx.2008.070178 | - |
dc.description.citedreference | Sasaki H, 2008, J CANCER RES CLIN, V134, P569, DOI 10.1007/s00432-007-0320-z | - |
dc.description.citedreference | Seok SH, 2008, KOREAN J PATHOL, V42, P94 | - |
dc.description.citedreference | Pinter F, 2008, J MOL DIAGN, V10, P160, DOI 10.2353/jmoldx.2008.070125 | - |
dc.description.citedreference | Hirsch FR, 2008, CANCER, V112, P1114, DOI 10.1002/cncr.23282 | - |
dc.description.citedreference | Yoo J, 2008, KOREAN J PATHOL, V42, P1 | - |
dc.description.citedreference | Lee S, 2007, KOREAN J PATHOL, V41, P387 | - |
dc.description.citedreference | Wei EX, 2007, MODERN PATHOL, V20, P905 | - |
dc.description.citedreference | Cappuzzo F, 2007, J CLIN ONCOL, V25, P2248, DOI 10.1200/JCO.2006.09.4300 | - |
dc.description.citedreference | Hirsch FR, 2007, ANN ONCOL, V18, P752, DOI 10.1093/annonc/mdm003 | - |
dc.description.citedreference | Jeon YK, 2006, LUNG CANCER, V54, P387, DOI 10.1016/j.lungcan.2006.08.015 | - |
dc.description.citedreference | Hirsch FR, 2006, J CLIN ONCOL, V24, P5034, DOI 10.1200/JCO.2006.06.3958 | - |
dc.description.citedreference | Italiano A, 2006, ANN ONCOL, V17, P981, DOI 10.1093/annonc/mdl038 | - |
dc.description.citedreference | Dacic S, 2006, AM J CLIN PATHOL, V125, P860, DOI 10.1309/H5UW6CPCWWC92241 | - |
dc.description.citedreference | Han SW, 2006, CLIN CANCER RES, V12, P2538, DOI 10.1158/1078-0432.CCR-05-2845 | - |
dc.description.citedreference | Nakamura H, 2006, THORAX, V61, P140, DOI 10.1136/thx.2005.042275 | - |
dc.description.citedreference | PRUDKIN L, 2006, ANN DIAGN PATHOL, V10, P306 | - |
dc.description.citedreference | DZIADZIUSZKO R, 2006, CLIN CANCER RES, V12, P4409 | - |
dc.description.citedreference | Takano T, 2005, J CLIN ONCOL, V23, P6829, DOI 10.1200/JCO.2005.01.0793 | - |
dc.description.citedreference | Hirsch FR, 2005, J CLIN ONCOL, V23, P6838, DOI 10.1200/JCO.2005.01.2823 | - |
dc.description.citedreference | Awaya H, 2005, J CLIN PATHOL, V58, P1076, DOI 10.1136/jcp.2004.025585 | - |
dc.description.citedreference | Taron M, 2005, CLIN CANCER RES, V11, P5878, DOI 10.1158/1078-0432.CCR-04-2618 | - |
dc.description.citedreference | Sequist LV, 2005, CLIN CANCER RES, V11, P5668, DOI 10.1158/1078-0432.CCR-05-1055 | - |
dc.description.citedreference | Shepherd FA, 2005, NEW ENGL J MED, V353, P123 | - |
dc.description.citedreference | Tsao MS, 2005, NEW ENGL J MED, V353, P133 | - |
dc.description.citedreference | Cappuzzo F, 2005, J NATL CANCER I, V97, P643, DOI 10.1093/jnci/dji112 | - |
dc.description.citedreference | Suzuki S, 2005, CANCER, V103, P1265, DOI 10.1002/cncr.20909 | - |
dc.description.citedreference | Tokumo M, 2005, CLIN CANCER RES, V11, P1167 | - |
dc.description.citedreference | Han SW, 2005, INT J CANCER, V113, P109, DOI 10.1002/ijc.20550 | - |
dc.description.citedreference | Pao W, 2004, P NATL ACAD SCI USA, V101, P13306, DOI 10.1073/pnas.0405220101 | - |
dc.description.citedreference | Ahn JH, 2004, J KOREAN MED SCI, V19, P529 | - |
dc.description.citedreference | Parra HS, 2004, BRIT J CANCER, V91, P208, DOI 10.1038/sj.bjc.6601923 | - |
dc.description.citedreference | Atkins D, 2004, J HISTOCHEM CYTOCHEM, V52, P893, DOI 10.1369/jhc.3A6195.2004 | - |
dc.description.citedreference | Paez JG, 2004, SCIENCE, V304, P1497, DOI 10.1126/science.1099314 | - |
dc.description.citedreference | Lynch TJ, 2004, NEW ENGL J MED, V350, P2129 | - |
dc.description.citedreference | Selvaggi G, 2004, ANN ONCOL, V15, P28, DOI 10.1093/annonc/mdh011 | - |
dc.description.citedreference | TRAVIS WD, 2004, WHO CLASSIFICATION T | - |
dc.description.citedreference | Niemiec J, 2004, FOLIA HISTOCHEM CYTO, V42, P111 | - |
dc.description.citedreference | Hirsch FR, 2003, J CLIN ONCOL, V21, P3798, DOI 10.1200/JCO.2003.11.069 | - |
dc.description.citedreference | Hirsch FR, 2003, LUNG CANCER-J IASLC, V41, pS29, DOI 10.1016/S0169-5002(03)00137-5 | - |
dc.description.citedreference | Meert AP, 2002, EUR RESPIR J, V20, P975, DOI 10.1183/09031936.02.00296502 | - |
dc.description.citedreference | Franklin WA, 2002, SEMIN ONCOL, V29, P3, DOI 10.1053/sonc.2002.31520 | - |
dc.description.citedreference | Nichols DK, 2001, J WILDLIFE DIS, V37, P1 | - |
dc.description.tc | 7 | - |
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