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Role of GnRH-GnRH receptor signaling at the maternal-fetal interface

Cited 12 time in Web of Science Cited 13 time in Scopus
Authors
Lee, Hee Joong; Snegovskikh, Victoria V.; Park, Joong Shin; Foyouzi, Nastaran; Hodgson, Eric J.; Norwitz, Errol R.; Guller, Seth; Han, Ku Taek
Issue Date
2010-12
Publisher
ELSEVIER SCIENCE INC
Citation
FERTILITY AND STERILITY; Vol.94 7; 2680-2687
Keywords
GnRHplacentadeciduahCGGnRH receptorcytokinespregnancyhuman
Abstract
Objective: To investigate the expression and function of GnRH and GnRH receptor (GnRHR) subtypes at the maternal-fetal interface. Design: In vitro experiments using freshly isolated human trophoblast cells, decidual stromal cells (DSCs), and immortalized cell lines. Setting: University teaching hospital. Patient(S): Placenta-fetal membranes from term deliveries. Intervention(s): Human trophoblast and DSCs were isolated, purified, and cultured. Main Outcome Measure(s): Expression of GnRH-I, GnRH-II, and GnRHR-I mRNA and protein in human trophoblast cell lines and tissues were evaluated by reverse-transcription polymerase chain reaction and Western blot. The effect of GnRH-I and -II on the production of select cytokines (hCG, interleukin [IL] 8, IL-6, matrix metalloproteinase 3, monocyte chemoattractant protein 1, vascular endothelial growth factor, soluble Fms-like tyrosine kinase 1, urokinase-type plasminogen activator, and plasminogen activator inhibitor 1) were measured by ELISA and normalized for protein content. Result(s): GnRH-I, GnRH-II, and GnRHR-I mRNA and protein were identified in trophoblasts and decidua. GnRH-I and -II stimulated hCG production by trophoblast and trophoblast-derived cell lines in a dose-dependent fashion (e. g., 2.8-fold, from 2.5 +/- 0.5 to 7.0 +/- 0.4 ng/mg protein per 24 h, for 1,000 nmol/L GnRH-I and 2.4-fold, from 2.5 +/- 0.5 to 6.1 +/- 0.6 ng/mg protein per 24 h, for 1,000 nmol/L GnRH-II) without affecting the production of other cytokines. Conclusion(s): Trophoblasts and decidua express GnRH-I, GnRH-II, and GnRHR-I mRNA and protein. GnRH-I and -II selectively stimulate hCG production by trophoblast cells without altering the production of select cytokines by trophoblasts or decidua. The role of GnRH-GnRHR signaling at the maternal-fetal interface therefore appears to be limited to the regulation of trophoblast hCG production. (Fertil Steril (R) 2010;94:2680-7. (C) 2010 by American Society for Reproductive Medicine.)
ISSN
0015-0282
Language
English
URI
https://hdl.handle.net/10371/76786
DOI
https://doi.org/10.1016/j.fertnstert.2010.03.016
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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